Label-Free Microfluidic Manipulation of Particles and Cells in Magnetic Liquids.
Label-Free Microfluidic Manipulation of Particles and Cells in Magnetic Liquids.
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DOI：
10.1002/adfm.201504178
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发表时间：
2016-06-14
影响因子：
19
通讯作者：
Mao L
中科院分区：
文献类型：
--
作者：
Zhao W;Cheng R;Miller JR;Mao L
在磁性液体中利用负磁泳效应操纵粒子和细胞是一个新的研究领域。它导致了微流体系统中的无标记和低成本操作技术以及许多令人兴奋的应用。本文介绍了负磁泳的基本原理及其在微流控技术中的应用。我们将首先讨论在磁性液体中的三个常用的操纵特性的理论背景，这三个特性包括颗粒和细胞的尺寸、密度和磁性。然后，我们将审查和比较介质中使用的负磁泳，其中包括顺磁性盐溶液和铁磁流体。之后，我们将重点回顾现有的负磁泳微流体应用，包括分离，聚焦，捕获和浓缩的颗粒和细胞，细胞密度的测定，测量颗粒的磁化率，和其他。我们还将研究开发用于活细胞操作和分析的生物相容性磁性液体的需求及其最新进展。最后，我们将结束这一审查与简要展望这一令人兴奋的研究领域。在磁性液体中操纵粒子和细胞是一个新的和令人兴奋的研究领域。它为微流体操作提供了无标签和低成本的技术。本文综述了它的基本原理和最新应用。
Manipulating particles and cells in magnetic liquids through so-called “negative magnetophoresis” is a new research field. It has resulted in label-free and low-cost manipulation techniques in microfluidic systems and many exciting applications. It is the goal of this review to introduce the fundamental principles of negative magnetophoresis and its recent applications in microfluidic manipulation of particles and cells. We will first discuss the theoretical background of three commonly used specificities of manipulation in magnetic liquids, which include the size, density and magnetic property of particles and cells. We will then review and compare the media used in negative magnetophoresis, which include paramagnetic salt solutions and ferrofluids. Afterwards, we will focus on reviewing existing microfluidic applications of negative magnetophoresis, including separation, focusing, trapping and concentration of particles and cells, determination of cell density, measurement of particles' magnetic susceptibility, and others. We will also examine the need for developing biocompatible magnetic liquids for live cell manipulation and analysis, and its recent progress. Finally, we will conclude this review with a brief outlook for this exciting research field. Manipulating particles and cells in magnetic liquids is a new and exciting research field. It offers label-free and low-cost techniques for microfluidic manipulation. Its fundamental principles and recent applications are reviewed.