To grow mouse mammary epithelial cells in culture.
To grow mouse mammary epithelial cells in culture.
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DOI：
10.1083/jcb.98.3.1026
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发表时间：
1984-03
期刊：
影响因子：
--
通讯作者：
Misfeldt DS
中科院分区：
文献类型：
--
作者：
Ehmann UK;Peterson WD Jr;Misfeldt DS
将Balb/c小鼠正常乳腺上皮细胞在经致毒照射的LA7饲养细胞的组织培养塑料上成功培养。饲养细胞还能促进单个小鼠乳腺细胞的集落形成，形成集落的细胞比例与饲养细胞的密度成正比。每次添加新的饲养细胞，小鼠乳腺细胞至少可传代8-12倍。现在培养的细胞数量增加了至少30倍，但体外寿命尚不清楚。通过这种技术维持的小鼠细胞培养从未过度生长成纤维细胞，并且在培养中形成了许多圆顶。
Normal mouse mammary epithelial cells from Balb/c mice were successfully cultivated on tissue culture plastic with lethally irradiated LA7 feeder cells. The feeder cells also promoted colony formation from single mouse mammary cells, and the fraction of cells that formed colonies was proportional to the density of feeder cells. The mouse mammary cells could be passaged at least 8-12 times as long as new feeder cells were added at each passage. The cells now in culture have doubled in number at least 30 times, but the in vitro lifespan is not yet known. The cultures of mouse cells maintained by this technique never became overgrown with fibroblasts and numerous domes formed in the cultures.