Topographical evaluation of human mesenchymal stem cells during osteogenic differentiation using scanning ion conductance microscopy
Topographical evaluation of human mesenchymal stem cells during osteogenic differentiation using scanning ion conductance microscopy
复制标题
DOI：
10.1016/j.electacta.2023.142192
复制
发表时间：
2023-03
影响因子：
6.6
通讯作者：
Kota Nozawa;Xuyang Zhang;Takuo Nakamura;Yuji Nashimoto;Yasufumi Takahashi;K. Ino;H. Shiku
中科院分区：
文献类型：
--
作者：
Kota Nozawa;Xuyang Zhang;Takuo Nakamura;Yuji Nashimoto;Yasufumi Takahashi;K. Ino;H. Shiku
人骨髓间充质干细胞（hMSCs）作为再生医学的重要细胞来源已引起广泛关注。成骨分化是hMSC的能力之一，对其临床应用至关重要。形态学测量对于监测hMSC成骨分化是必不可少的，因为它们是非破坏性的，并且保存了有价值的细胞来源。然而，在分化过程中的细胞的三维形貌还没有得到充分的研究。在这里，我们定量的时间过程中的变化，hMSC地形成骨分化，使用扫描离子电导显微镜（SICM）。快速扩增克隆（REC），具有高增殖和分化潜能的hMSC亚群，和常规hMSC（cMSC）被用作hMSC来源。在成骨分化培养基中，RECs和cMSCs的细胞高度低于未分化培养基中的细胞高度，表明在成骨分化过程中发生了形态学变化。有趣的是，在分化过程中，RECs在培养期开始时（第1天）表现出较低的高度，而cMSCs的高度逐渐降低，这意味着RECs具有比cMSCs更高的分化能力。三维形貌信息将有助于更深入地了解hMSC的成骨分化，并可能成为未来临床应用的重要分化指标。
Human mesenchymal stem cells (hMSCs) have attracted considerable attention as valuable cell sources for regenerative medicine in various diseases. Osteogenic differentiation is one of the capabilities of hMSCs that is critical to their clinical applications. Morphological measurements are essential for monitoring hMSC osteogenic differentiation because they are non-destructive and conserve valuable cell sources. However, the three-dimensional topography of cells during differentiation has not been fully investigated. Here, we quantified the time-course changes in hMSC topography during osteogenic differentiation using scanning ion conductance microscopy (SICM). Rapidly expanding clone (REC), an hMSC subpopulation with high proliferation and differentiation potencies, and conventional hMSCs (cMSCs) were used as the hMSC sources. In the osteogenic differentiation medium, RECs and cMSCs showed lower cell heights than those in the undifferentiation medium, indicating the occurrence of morphological changes during the osteogenic differentiation. Interestingly, during differentiation, RECs exhibited a low height at the beginning of the culture period (day 1), while the height of cMSCs gradually decreased, implying that RECs had a higher differentiation capacity than cMSCs. The three-dimensional topographical information will contribute to a deeper understanding of hMSC osteogenic differentiation and may become an important differentiation indicator in future clinical applications.