Efficacy of clone fingerprinting methodologies
Efficacy of clone fingerprinting methodologies
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DOI：
10.1016/j.ygeno.2006.08.008
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发表时间：
2007-01-01
期刊：
影响因子：
4.4
通讯作者：
Soderlund, Carol
中科院分区：
文献类型：
--
作者：
Nelson, William M.;Dvorak, Jan;Soderlund, Carol
随着用于构建基于BAC的物理地图的新的高信息含量指纹技术的发展，物理地图构建正在加速，并且确定哪种方法最有效是很重要的。在最近的一份出版物（Z。Xu等人，2004，Genomics 84：941-951），Xu等人评估了五种不同的技术（一种基于琼脂糖，四种使用多种酶），并得出结论，双酶技术是上级的。此外，他们发现，超过10倍的指纹覆盖率没有任何好处。在本文中，我们报告我们自己的广泛的模拟结果，从而导致对比的结论。我们的数据表明，被称为SNaPshot的五酶方法是最有效的，并且事实上可以在大于10倍覆盖率的情况下显着改善组装。(c)2006年爱思唯尔公司All rights reserved.
With the development of new high-information content fingerprinting techniques for constructing BAC-based physical maps, physical map construction is accelerating and it is important to determine which methodologies work best. In a recent publication (Z. Xu et al., 2004, Genomics 84:941-951), Xu et al. evaluated five different techniques (one agarose-based and four using multiple enzymes) and concluded that a two-enzyme technique was superior. In addition, they found that no benefit was gained from fingerprinting more than 10x coverage. In this paper we report our own extensive simulation results, which lead to contrasting conclusions. Our data indicate that the five-enzyme method known as SNaPshot is the most effective and that the assembly can in fact be significantly improved with greater than 10x coverage. (c) 2006 Elsevier Inc. All rights reserved.