Improved in vitro cultivation of Babesia caballi
Improved in vitro cultivation of Babesia caballi
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DOI：
10.1292/jvms.59.479
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发表时间：
1997-06-01
影响因子：
1.2
通讯作者：
Suzuki, N
中科院分区：
文献类型：
--
作者：
Avarzed, A;Igarashi, I;Suzuki, N
从实验性感染的马的血液中收集卡巴利巴伯氏线虫感染的红细胞，并且可以在低氧气氛（2%O-2、5%CO2和93%N-2）中在补充有2 mM L-谷氨酰胺、20 mM HEPES和40%成年马血清的RPMI 1640培养基中在寄生虫血症范围为2-4%的情况下在体外连续培养。使用其他培养基、血清浓度和培养容器增加寄生虫血症的所有尝试均失败。然而，寄生虫的生长增强了从低氧到5%CO2在空气中的培养物的转移，寄生虫血症范围从8- 10%。
Babesia caballi infected erythrocytes were collected from the blood of an experimentally infected horse and could be continuously cultivated in vitro with parasitemia ranging from 2-4% in RPMI 1640 medium supplemented with 2 mM L-glutamine, 20 mM HEPES and 40% adult horse serum in a low oxygen atmosphere (2% O-2, 5% CO2 and 93% N-2. All attempts to increase parasitemia failed using other culture media, serum concentrations and culture vessels. However, parasite growth was enhanced by transfer of cultures from a low oxygen to 5% CO2 in air, with parasitemia ranging from 8-10%.