SELF-CLEAVAGE OF VIRUSOID RNA IS PERFORMED BY THE PROPOSED 55-NUCLEOTIDE ACTIVE-SITE
SELF-CLEAVAGE OF VIRUSOID RNA IS PERFORMED BY THE PROPOSED 55-NUCLEOTIDE ACTIVE-SITE
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DOI：
10.1016/0092-8674(87)90657-x
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发表时间：
1987-07-03
期刊：
影响因子：
64.5
通讯作者：
SYMONS, RH
中科院分区：
文献类型：
--
作者：
FORSTER, AC;SYMONS, RH
类病毒是依赖于植物病毒进行复制和降解的环状单链RNA。最近，我们发现体外合成的含有324个核苷酸的卢塞恩瞬时线条病毒类病毒的273个核苷酸的RNA在一个独特的位点自我切割。该反应需要加热和快速冷却RNA，随后加入镁离子。在这里，我们通过制备具有5“和3”末端缺失的RNA来测试为活性位点提出的55个核苷酸的锤头状结构模型。结果表明，锤头结构是足够的和必要的自我切割，某些序列防止形成锤头结构的天然类病毒RNA，和RNA分子仅含有52个核苷酸是能够RNA介导的反应。
Virusoids are circular single-stranded RNAs dependent on plant viruses for replication and encapsidation. Recently, we showed that an in vitro-synthesized RNA containing 273 nucleotides of the 324-nucleotide virusoid of lucerne transient streak virus self-cleaves at a unique sites. The reaction requires heating and snap cooling of the RNA and the subsequent addition of magnesium ions. Here, we test the 55-nucleotide, hammerhead-shaped, structural model proposed for the active site by preparing RNAs with both 5'' and 3'' terminal deletions. Results indicate that the hammer-head structure is sufficient and necessary for self-cleavage, that certain sequences prevent the formation of the hammerhead structure in the native virusoid RNA, and that an RNA molecule containing only 52 nucleotides is capable of an RNA-mediated reaction.