Population Analysis of Structural Properties of Giant Liposomes by Flow Cytometry
Population Analysis of Structural Properties of Giant Liposomes by Flow Cytometry
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DOI：
10.1021/la902237y
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发表时间：
2009-09-15
期刊：
影响因子：
3.9
通讯作者：
Yomo, Tetsuya
中科院分区：
文献类型：
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作者：
Nishimura, Kazuya;Hosoi, Tomohiro;Yomo, Tetsuya
我们使用荧光流式细胞仪分析了不同制备方法形成的巨型脂质体群体的结构特性。使用荧光标记同时测量大量单个脂质体的内部水体积和标称膜表面积。我们比较了自然溶胀法、冻干空脂质体法和油包水（W/O）乳液法制备的脂质体的这些性质。使用内部体积和标称表面积的二维轮廓分布图来阐明各种脂质体尺寸的脂质体的结构特性。脂质体的层状评价为标称表面积与根据脂质体内部体积计算的理论表面积的比率。该群体分析揭示了层状对脂质体体积的依赖性：自然溶胀法和冻干空脂质体法制备的脂质体群体的标称表面积分布广泛，而W/O乳液法制备的脂质体群体的标称表面积分布较窄，数值较小。此外，使用后一种方法，标称表面积与内部体积的三分之二次方成比例变化，范围为几个数量级，表明脂质体具有薄膜，该薄膜在宽体积范围内是恒定的。这里提出的结果以及方法将有助于设计具有所需特性的巨型脂质体。
We used fluorescence flow cycometry to analyze the structural properties of populations of giant liposomes formed by different preparation methods. The inner aqueous volumes and nominal membrane surface areas of a large number of individual liposomes were measured simultaneously by using fluorescent markers. We compared these properties of liposomes prepared by the natural swelling method, the freeze-dried empty liposomes method, and the water-in-oil (W/O) emulsion method. A two-dimensional contour distribution map of the inner volume and the nominal surface area was used to elucidate the structural properties of liposomes over a wide range of liposome sizes. Lamellarity of liposomes was evaluated as the ratio of the nominal surface area to the theoretical surface area calculated from the liposome inner volume. This population analysis revealed the dependency of lamellarity on liposome volume: while the nominal surface areas of populations of liposomes prepared by the natural swelling and the freeze-dried empty liposome methods were widely distributed, those prepared by the W/O emulsion method had a narrower distribution within small values. Furthermore, with the latter method, the nominal surface area varied in proportion to the two-thirds power of the inner volume ranging for several orders of magnitude, indicating the liposomes had a thin membrane, which was constant for the wide Volume range. The results as well as the methodology presented here would be useful in designing giant liposomes with desired properties.