Differentiation and Characterization of Tr1 Cells.
Differentiation and Characterization of Tr1 Cells.
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DOI：
10.1002/0471142735.im0327s113
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发表时间：
2016-04-01
影响因子：
--
通讯作者：
Kuchroo VK
中科院分区：
文献类型：
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作者：
Chihara N;Madi A;Karwacz K;Awasthi A;Kuchroo VK
调节性T细胞介导的抑制是免疫介导的炎症负调节的关键机制。1型调节性T细胞（Tr 1细胞）是CD 4 + T细胞的重要亚群，有助于控制过度的炎症反应和维持耐受性。Tr 1细胞介导抗炎作用的一种方式是通过它们产生白细胞介素10（IL-10），其抑制抗原呈递细胞和抗原特异性效应T细胞的功能。此外，Tr 1细胞能够通过穿孔蛋白-粒酶B途径杀死效应细胞和骨髓细胞。体外分化的Tr 1细胞在体内过继转移后抑制自身免疫组织炎症的发展。在这里，我们描述了在体外分化的幼稚小鼠CD 4 + T细胞的IL-10产生的Tr 1细胞通过使用IL-27刺激。
Regulatory T cells mediated suppression serves pivotal mechanism of negative-regulation of immune mediated inflammation. Type 1 regulatory T cells (Tr1 cells) are an important subset of CD4+ T cells that help to control excessive inflammatory responses and maintain tolerance. One way in which Tr1 cells mediate an anti-inflammatory role is via their production of interleukin 10 (IL-10), which dampens the function of both antigen-presenting cells and antigen-specific effector T cells. Additionally, Tr1 cells are capable of killing effector and myeloid cells through the Perforin-Granzyme B pathway. In vitro differentiated Tr1 cells suppress development of autoimmune tissue inflammation upon adoptive transfer in vivo. Here we describe the in vitro differentiation of naïve murine CD4+ T cells to IL-10 producing Tr1 cells by using IL-27 stimulation.