DIFFERENTIAL EXPRESSION OF THE 2 VEGF RECEPTORS FLT AND KDR IN PLACENTA AND VASCULAR ENDOTHELIAL-CELLS
DIFFERENTIAL EXPRESSION OF THE 2 VEGF RECEPTORS FLT AND KDR IN PLACENTA AND VASCULAR ENDOTHELIAL-CELLS
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DOI：
10.1002/jcb.240540107
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发表时间：
1994-01-01
影响因子：
4
通讯作者：
WEICH, HA
中科院分区：
文献类型：
--
作者：
BARLEON, B;HAUSER, S;WEICH, HA
血管内皮生长因子（VEGF）是一种新发现的对内皮细胞具有特异性的生长和通透性因子。最近，fit编码的酪氨酸激酶被表征为VEGF的受体。一种新的酪氨酸激酶受体编码的KDR基因也被发现结合VEGF的高亲和力时，在CMT-3细胞中表达。在多种物种和组织来源的内皮细胞中筛选fit和KDR表达表明fit主要在人胎盘和人血管内皮细胞中表达。胎盘生长因子（PlGF）是一种与VEGF密切相关的生长因子，在胎盘和人血管内皮细胞中与fit共表达。KDR在所有血管源性内皮细胞中分布和表达更广泛。这些数据表明，从不同组织分离的培养的人内皮细胞以相对高的水平表达VEGF受体，此外，培养物中所有研究的非人内皮细胞也对KDR基因表达呈阳性。(C)1994 Wiley-Liss，Inc.
Vascular endothelial growth factor (VEGF) is a newly identified growth and permeability factor with a unique specificity for endothelial cells. Recently the fit-encoded tyrosine kinase was characterized as a receptor for VEGF. A novel tyrosine kinase receptor encoded by the KDR gene was also found to bind VEGF with high affinity when expressed in CMT-3 cells. Screening for fit and KDR expression in a variety of species and tissue-derived endothelial cells demonstrates that fit is predominantly expressed in human placenta and human vascular endothelial cells. Placenta growth factor (PlGF), a growth factor significantly related to VEGF, is coexpressed with fit in placenta and human vascular endothelial cells. KDR is more widely distributed and expressed in all vessel-derived endothelial cells. These data demonstrate that cultured human endothelial cells isolated from different tissues express both VEGF receptors in relative high levels and, additionally, that all investigated nonhuman endothelial cells in culture are also positive for KDR gene expression. (C) 1994 Wiley-Liss, Inc.