Concepts in imaging and microscopy -: Imaging membrane potential with voltage-sensitive dyes
Concepts in imaging and microscopy -: Imaging membrane potential with voltage-sensitive dyes
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DOI：
10.2307/1542798
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发表时间：
2000-02-01
影响因子：
1.6
通讯作者：
Zecevic, D
中科院分区：
文献类型：
--
作者：
Zochowski, M;Wachowiak, M;Zecevic, D
膜电位可以使用多种分子探针进行光学测量。这些测量可以用于在单个细胞水平上研究功能，用于确定神经元组如何产生行为，以及用于研究神经元群体的相关行为。给出了这三种测量方法的实例。从这些测量中获得的信号通常很小。必要的方法论考虑，以优化所得的信噪比进行了讨论。
Membrane potential can be measured optically using a variety of molecular probes. These measurements can be useful in studying function at the level of an individual cell, for determining how groups of neurons generate a behavior, and for studying the correlated behavior of populations of neurons. Examples of the three kinds of measurements are presented. The signals obtained from these measurements are generally small. Methodological considerations necessary to optimize the resulting signal-to-noise ratio are discussed.