DETECTION OF SINGLE MOLECULES OF PHYCOERYTHRIN IN HYDRODYNAMICALLY FOCUSED FLOWS BY LASER-INDUCED FLUORESCENCE
DETECTION OF SINGLE MOLECULES OF PHYCOERYTHRIN IN HYDRODYNAMICALLY FOCUSED FLOWS BY LASER-INDUCED FLUORESCENCE
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DOI：
10.1021/ac00144a032
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发表时间：
1987-09-01
影响因子：
7.4
通讯作者：
MARTIN, JC
中科院分区：
文献类型：
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作者：
NGUYEN, DC;KELLER, RA;MARTIN, JC
通过激光诱导荧光在流体动力学聚焦流中检测B-藻红蛋白的单分子，因为每个分子通过聚焦的激光束。藻红蛋白是一种含有25个罗丹明-6G发色团的大蛋白分子。单分子检测记录如下：（1）每个分子的计数数与预期数一致，（2）每秒的分子数与浓度和流速一致，（3）检测到的分子之间的时间间隔分布与浓度和流速一致。分子通过1.1-pL探针体积的通过时间为180 μ s。
Single molecules of B-phycoerythrin were detected by laser-induced fluorescence in hydrodynamically focused flows as each molecule transited a focused laser beam. Phycoerythrin is a large protein molecule containing the equivalent of 25 rhodamine-6G chromophores. Single molecule detection is documented by the following: (1) the number of counts per molecules is in agreement with the expected number, (2) the number of molecules per second is in agreement with the concentration and the flow rate, and (3) the time interval distribution between detected molecules is in agreement with the concentration and the flow rate. The molecular transit time through the 1.1-pL probe volume was 180 .mu.s.