Thermal Fluctuation and Elasticity of Lipid Vesicles Interacting with Pore-Forming Peptides
Thermal Fluctuation and Elasticity of Lipid Vesicles Interacting with Pore-Forming Peptides
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DOI：
10.1103/physrevlett.105.038101
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发表时间：
2010-07-13
影响因子：
8.6
通讯作者：
Kline, Steven R.
中科院分区：
文献类型：
--
作者：
Lee, Ji-Hwan;Choi, Sung-Min;Kline, Steven R.
通过中子自旋回波测量研究了二油酰磷酸胆碱大单层囊泡与成孔肽蜂毒肽相互作用的热波动和弹性。不同肽脂摩尔比 P/L 下膜波动的弛豫行为可分为三个区域，这是由于膜的有效弯曲模量 (kappa) 随波浪线变化的特征变化而产生的，其中包括膜内内部耗散的影响。在低 P/L 时，蜂毒肽平行于膜表面吸附，并且由于烃链堆积的扰动，波浪号上的 (kappa) 显着降低。在临界 P/L 处，蜂毒肽在膜中形成孔，并且由于高孔刚性，波浪线上方的 (kappa) 开始略有增加。在较高的 P/L 下，排斥性孔间相互作用变得显着，波浪号上的 (kappa) 迅速增加。
The thermal fluctuation and elasticity of dioleoyl-phosphocholine large unilamellar vesicle interacting with pore-forming peptide, melittin, were investigated by neutron spin-echo measurements. The relaxation behavior of the membrane fluctuation with different peptide to lipid molar ratio P/L can be divided into three regions, resulting from characteristic changes of the effective bending modulus (kappa) over tilde of the membrane which includes the effects of internal dissipation within the membrane. At low P/L, melittin is adsorbed parallel to the surface of membrane and (kappa) over tilde decreases significantly due to perturbation of hydrocarbon chain packing. At a critical P/L, melittin forms pores in the membrane and (kappa) over tilde starts to increase slightly due to high pore rigidity. At higher P/L where the repulsive interpore interaction becomes significant, (kappa) over tilde increases rapidly.