Luteolin, a flavonoid, inhibits CD40 ligand expression by activated human basophils
Luteolin, a flavonoid, inhibits CD40 ligand expression by activated human basophils
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DOI：
10.1159/000092554
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发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Tanaka, Toshio
中科院分区：
文献类型：
--
作者：
Hirano, Toru;Arimitsu, Junsuke;Tanaka, Toshio
背景我们以前已经表明，黄酮类化合物，如毛地黄黄酮，芹菜素和非瑟酮抑制白细胞介素4和白细胞介素13的生产。在这项研究中，我们研究了毛地黄黄酮是否可以抑制CD40配体表达的嗜碱性粒细胞。研究方法：人嗜碱性细胞系KU812，刺激与A23187和佛波醇肉豆蔻酸酯乙酸酯（PMA）与或没有不同浓度的毛地黄黄酮或其他黄酮类化合物12小时，和CD40配体表达进行了分析，通过流式细胞仪。用半定量逆转录PCR方法研究木犀草素对CD40配体mRNA表达的影响。此外，CD40配体的表达也测定了纯化的嗜碱性粒细胞，已刺激12 In与A23187加PMA与或没有不同浓度的毛地黄黄酮。结果：A23187可显著增强KU812细胞表达CD40配体，A23187与PMA联用更能显著增强KU812细胞表达CD40配体。表达被10或30 μ M的毛地黄黄酮显着抑制，而杨梅素未能抑制。逆转录PCR分析表明，毛地黄黄酮抑制刺激的KU812细胞CD40配体mRNA的表达。六个类黄酮检查，毛地黄黄酮，芹菜素，非瑟酮和槲皮素在30 μ M显示出显着的抑制作用，CD40配体的表达。纯化的嗜碱性粒细胞与A23187加PMA的孵育显着增强CD40配体的表达，并且毛地黄黄酮的存在再次具有抑制作用。结论：木犀草素抑制活化的嗜碱性粒细胞表达CD40配体。版权所有（c）2006 S. Karger AG，巴塞尔。
Background. We have previously shown that flavonoids such as luteolin, apigenin and fisetin inhibit interleukin 4 and interleukin 13 production. In this study, we investigated whether luteolin can suppress CD40 ligand expression by basophils. Methods: A human basophilic cell line, KU812, was stimulated with A23187 and phorbol myristate acetate (PMA) with or without various concentrations of luteolin or other flavonoids for 12 h, and CD40 ligand expression was analyzed by FACS. The effect of luteolin on CD40 ligand mRNA expression was studied by semiquantitative reverse transcription PCR analysis. In addition, CD40 ligand expression was also measured in purified basophils that had been stimulated for 12 In with A23187 plus PMA with or without various concentrations of luteolin. Results: CD40 ligand expression by KU812 cells was enhanced noticeably in response to A23187 and even more strikingly augmented by A23187 plus PMA. The expression was significantly suppressed by 10 or 30 mu M of luteolin, whereas myricetin failed to inhibit. Reverse transcription PCR analyses demonstrated that luteolin inhibited CD40 ligand mRNA expression by stimulated KU812 cells. Of the six flavonoids examined, luteolin, apigenin, fisetin and quercetin at 30 mu M showed a significant inhibitory effect on CD40 ligand expression. The incubation of purified basophils with A23187 plus PMA significantly enhanced CD40 ligand expression, and the presence of luteolin again had an inhibitory effect. Conclusions: Luteolin inhibits CD40 ligand expression by activated basophils. Copyright (c) 2006 S. Karger AG, Basel.