Individual CLA Isomers, c9t11 and t10c12, Prevent Excess Liver Glycogen Storage and Inhibit Lipogenic Genes Expression Induced by High-Fructose Diet in Rats.
Individual CLA Isomers, c9t11 and t10c12, Prevent Excess Liver Glycogen Storage and Inhibit Lipogenic Genes Expression Induced by High-Fructose Diet in Rats.
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DOI：
10.1155/2015/535982
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发表时间：
2015
影响因子：
--
通讯作者：
Kostogrys RB
中科院分区：
文献类型：
--
作者：
Maslak E;Buczek E;Szumny A;Szczepnski W;Franczyk-Zarow M;Kopec A;Chlopicki S;Leszczynska T;Kostogrys RB
本研究评估了单个共轭亚油酸异构体c9 t11-CLA和t10 c12-CLA对大鼠非酒精性脂肪肝（NAFLD）和全身内皮功能障碍的影响，这些大鼠用对照或高果糖饮食喂养四周。高果糖饮食阻碍体重增加（不影响食物摄入），增加肝脏重量和肝细胞中的糖原储存，上调脂肪酸合成酶（FAS）和硬脂酰辅酶A去饱和酶-1（SCD-1）的表达，并增加肝脏中的饱和脂肪酸（SFA）含量。这两种CLA异构体都能防止肝脏中糖原的过度积累。具体而言，t10 c12-CLA降低血清甘油三酯和LDL + VLDL胆固醇的浓度，增加HDL胆固醇，并通过下调肝脏SCD-1和FAS表达影响肝脏脂质含量和脂肪酸组成。反过来，c9 t11-CLA降低了对照组的LDL+VLDL胆固醇，下调了FAS的肝脏表达，但对肝脏重量、脂质含量和脂肪酸组成没有显著影响。总之，用高果糖饮食喂养大鼠导致肝糖原储存增加，表明尽管参与从头脂肪生成的基因同时上调，但仍诱导了脂肪生成。虽然这两种CLA异构体（c9 t11和t10 c12）显示保肝活性，降脂作用的t10 c12-CLA异构体被证明是更明显的比c9 t11-CLA。
This study assessed the effects of individual conjugated linoleic acid isomers, c9t11-CLA and t10c12-CLA, on nonalcoholic fatty liver disease (NAFLD) and systemic endothelial dysfunction in rats fed for four weeks with control or high-fructose diet. The high-fructose diet hampered body weight gain (without influencing food intake), increased liver weight and glycogen storage in hepatocytes, upregulated expression of fatty acid synthase (FAS) and stearoyl-CoA desaturase-1 (SCD-1), and increased saturated fatty acid (SFA) content in the liver. Both CLA isomers prevented excessive accumulation of glycogen in the liver. Specifically, t10c12-CLA decreased concentration of serum triacylglycerols and LDL + VLDL cholesterol, increased HDL cholesterol, and affected liver lipid content and fatty acid composition by downregulation of liver SCD-1 and FAS expression. In turn, the c9t11-CLA decreased LDL+VLDL cholesterol in the control group and downregulated liver expression of FAS without significant effects on liver weight, lipid content, and fatty acid composition. In summary, feeding rats with a high-fructose diet resulted in increased liver glycogen storage, indicating the induction of gluconeogenesis despite simultaneous upregulation of genes involved in de novo lipogenesis. Although both CLA isomers (c9t11 and t10c12) display hepatoprotective activity, the hypolipemic action of the t10c12-CLA isomer proved to be more pronounced than that of c9t11-CLA.
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影响因子：
5.1
作者：
Lee, O.;Bruce, W. R.;O'Brien, P. J.
通讯作者：
O'Brien, P. J.
影响因子：
6
作者：
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DOI：
10.1152/ajpendo.00293.2009
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2009-12-01
影响因子：
5.1
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6.9
作者：
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通讯作者：
Al-Mohanna, Futwan A.