Calcium and zinc decrease intracellular iron by decreasing transport during iron repletion in an in vitro model
Calcium and zinc decrease intracellular iron by decreasing transport during iron repletion in an in vitro model
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DOI：
10.1007/s00394-017-1535-7
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发表时间：
2018-12-01
影响因子：
5
通讯作者：
Miguel, Arredondo
中科院分区：
文献类型：
--
作者：
Monica, Andrews;Lautaro, Briones;Miguel, Arredondo
目的铁是一种必需的微量营养素，参与多种生命反应，其吸收可受多种营养因素的影响，如其他微量营养素。我们的假设是，由于与锌和钙的相互作用，铁的吸收减少。方法用1 mM钙、10、30 mU M-锌和/或10、20、30 mU M-Fe培养Caco-2细胞24 h，观察钙和锌的相互作用对细胞铁摄取和转运、细胞内Fe和锌水平以及DMT1、铁蛋白、ZIP4和ZnT1基因表达的影响。在钙、锌、铁处理下，锌的含量增加最多。随着细胞内铁离子浓度的增加，DMT1mRNA的表达下降。不同铁浓度培养的细胞中铁转运蛋白的表达无明显变化。钙、锌和铁的混合作用主要在高锌浓度下增加DMT1和铁孔蛋白的表达。钙和铁主要促进ZIP4的表达，但在所研究的所有条件下，ZIP4的表达没有变化。与对照细胞相比，所有研究条件下的细胞对铁的摄取都更高；然而，只有在单独加入铁的细胞中，铁的转运增加。在其他条件下，铁的转运均低于对照细胞。结论钙、锌干扰铁代谢。这种干扰是通过增加铁蛋白的活性来实现的，这会导致铁的净吸收减少。
PurposeIron is an essential micronutrient that participates in a number of vital reactions and its absorption may be altered by various nutritional factors such as other micronutrients. Our hypothesis is that iron absorption is decreased because of the interactions with zinc and calcium. We evaluated the interaction between calcium and zinc on iron uptake and transport, intracellular Fe and Zn levels and mRNA expression of DMT1, ferroportin, Zip4 and ZnT1 in an in vitro model.Methods Caco-2 cells were cultivated with 1 mM Ca; 10 or 30 mu M Zn and/or 10, 20 or 30 mu M Fe for 24 h.Results Intracellular Fe decreased in cells incubated with 30 mu M Zn or with the mix Ca/10 mu M Zn/Fe. Zn mostly increased under Ca, Zn and Fe treatment. DMT1 mRNA expression decreased when intracellular Fe increased. Ferroportin expression displayed no change in cells cultured with different Fe concentrations. The mix of Ca, Zn and Fe increased DMT1 and ferroportin expression mainly under high Zn concentration. Zip4 expression was mostly augmented by Ca and Fe; however, ZnT1 showed no change in all conditions studied. Fe uptake was higher in all the conditions studied compared to control cells; however, Fe transport increased only in cells incubated with Fe alone. In all the other conditions, Fe transport was lower than that in control cells.Conclusions The present findings suggest that Ca and Zn interfere with iron metabolism. This interference is through an increase in ferroportin activity, which results in a diminished net iron absorption.