DIFFERENTIAL DISTRIBUTION OF BETA-TUBULIN ISOTYPES IN CEREBELLUM
DIFFERENTIAL DISTRIBUTION OF BETA-TUBULIN ISOTYPES IN CEREBELLUM
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DOI：
10.1002/j.1460-2075.1988.tb03074.x
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发表时间：
1988-08-01
期刊：
影响因子：
11.4
通讯作者：
COWAN, NJ
中科院分区：
文献类型：
--
作者：
BURGOYNE, RD;CAMBRAYDEAKIN, MA;COWAN, NJ
我们描述了哺乳动物β蛋白的结构和表达。-微管蛋白同型（m.b a.6），在睾丸中表达较弱，但在发育中的大脑中表达丰富，在成人大脑中转录量下降到较低水平。m的表达式。6在其他小鼠组织中检测不到。用克隆的融合蛋白作为免疫原制备特异性血清。M.beta。6是已知的5个beta之一。-微管蛋白同型在大脑中表达，并使用抗m。血清和血清，抗m。2, anti-M.beta。3/4和抗m。5，在之前的研究中，我们研究了β的表达模式。大鼠小脑-微管蛋白同型。每种同型在小脑中都有特定的细胞类型定位模式。M.beta。2, M.beta。3/4和m。在神经元细胞和非神经元细胞中均存在，但与之相反，m.b。6仅在组织切片和分离的小脑细胞培养的神经元中检测到。. β。-微管蛋白同型位于羧基端，即- β区域。-微管蛋白参与MAP结合。在m。2和m。6、表达模式分别与MAP3和MAP1A的表达模式相似或相同。这些结果表明。-微管蛋白同型可能有助于确定MAPs与不同功能微管的特定关联。然而，脑内其他map的严格亚细胞分离可能是由其他因素决定的。
We describe the structure and expression of a mammalian .beta.-tubulin isotype (M.beta.6) that is weakly expressed in testis but is abundant in developing brain, with transcripts declining to lower levels in the adult brain. The expression of M.beta.6 was undetectable in any other mouse tissue examined. A serum specific for this isotype was prepared using a cloned fusion protein as immunogen. M.beta.6 is one of five known .beta.-tubulin isotypes expressed in brain, and using the anti-M.beta.6 serum along with sera, anti-M.beta.2, anti-M.beta.3/4 and anti-M.beta.5, previously characterized, we have examined the pattern of expression of .beta.-tubulin isotypes in rat cerebellum. The isotypes each have characteristic cell-type specific patterns of localization in cerebellum. M.beta.2, M.beta.3/4 and M.beta.5 are present in both neuronal and non-neuronal cells, but in contrast M.beta.6 was only detectable in neurons in tissue sections and in dissociated cerebellar cell culture. The majority of sequence differences among the .beta.-tubulin isotypes lie at the carboxy terminus, the region of .beta.-tubulin involved in MAP binding. In the case of M.beta.2 and M.beta.6, the patterns of expression are similar or identical to the patterns of expression of MAP3 and MAP1A respectively. These results suggest that .beta.-tubulin isotypes may contribute to the determination of the specific association of MAPs with microtubules of diverse function. However, the strict subcellular segregation of other MAPs in brain may be determined by other factors.