Harnessing yeast organelles for metabolic engineering
Harnessing yeast organelles for metabolic engineering
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DOI：
10.1038/nchembio.2429
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发表时间：
2017-08-01
影响因子：
14.8
通讯作者：
Avalos, Jose L.
中科院分区：
文献类型：
--
作者：
Hammer, Sarah K.;Avalos, Jose L.
酵母中的每个亚细胞区室提供了独特的生理化学环境和代谢物、酶和辅因子组成。虽然酵母代谢工程的重点是在细胞胞质溶胶中组装途径，但对亚细胞区室化的兴趣越来越大。除了利用不同的细胞器特性之外，细胞器与胞质溶胶的物理分离具有消除代谢串扰和增强区室化途径效率的潜力。在这个角度来看，我们回顾了酵母亚细胞工程的最新技术水平，突出了针对亚细胞区室，包括线粒体，过氧化物酶体，ER和/或高尔基体，液泡和细胞壁，在不同的酵母物种的生物合成途径的好处。我们比较了与亚细胞工程开发的菌株的性能，以那些本地生产者或酵母菌株以前与胞质途径工程。我们还确定了未来的重要挑战，需要解决细胞器工程成为学术界和工业界胞质工程的主流。
Each subcellular compartment in yeast offers a unique physiochemical environment and metabolite, enzyme, and cofactor composition. While yeast metabolic engineering has focused on assembling pathways in the cell cytosol, there is growing interest in embracing subcellular compartmentalization. Beyond harnessing distinct organelle properties, physical separation of organelles from the cytosol has the potential to eliminate metabolic crosstalk and enhance compartmentalized pathway efficiency. In this Perspective we review the state of the art in yeast subcellular engineering, highlighting the benefits of targeting biosynthetic pathways to subcellular compartments, including mitochondria, peroxisomes, the ER and/or Golgi, vacuoles, and the cell wall, in different yeast species. We compare the performances of strains developed with subcellular engineering to those of native producers or yeast strains previously engineered with cytosolic pathways. We also identify important challenges that lie ahead, which need to be addressed for organelle engineering to become as mainstream as cytosolic engineering in academia and industry.