Undibacterium baiyunense sp. nov., Undibacterium curvum sp. nov., Undibacterium fentianense sp. nov., Undibacterium flavidum sp. nov., Undibacterium griseum sp. nov., Undibacterium hunanense sp. nov., Undibacterium luofuense sp. nov., Undibacterium nitidu
Undibacterium baiyunense sp. nov., Undibacterium curvum sp. nov., Undibacterium fentianense sp. nov., Undibacterium flavidum sp. nov., Undibacterium griseum sp. nov., Undibacterium hunanense sp. nov., Undibacterium luofuense sp. nov., Undibacterium nitidu
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白云Undibacteria sp.
DOI：
10.1099/ijsem.0.005065
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发表时间：
2021
影响因子：
2.8
通讯作者：
Xu Meiying
中科院分区：
文献类型：
--
作者：
Lu Huibin;Liu Fei;Deng Tongchu;Xu Meiying
从中国河流中分离到12株革兰氏阴性、过氧化氢酶和氧化酶阳性、杆状、运动型的菌株（CY 7 WT、CY 18 WT、CY 22 WT、FT 31 WT、FT 137 WT、FT 147 WT、BYS 50 W、BYS 107 WT、LFS 511 WT、LX 15 WT、LX 22 WT和NL 8 WT）。根据16 S rRNA基因序列的比较表明，这些菌株以Undibacterium属的物种为近邻。构建的系统发育树和基因组进化树也表明这些菌株与Undibacterium属的种聚在一起。这些菌株的基因组G+C含量在45.3 ~ 53.3mol%之间。除CY 7 WT和BYS 50 W菌株间的OrthoANIu值分别为99.0%和91.8%外，其余菌株间的OrthoANIu值和DNA-DNA杂交值分别为70.4%~ 94.1%和19.3%~ 55.3%。 Q-8是它们的主要呼吸醌。C16：1ω 7 c和C16：0为主要脂肪酸。 它们的极性脂谱相似，包括磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的磷脂和两种未鉴定的氨基脂。结合多相分类学特征和系统发育关系，12株菌应代表11个独立的新属种，命名为白云Undibacterium baiyunensep. nov.（模式菌株BYS 107 WT =GDMCC 1.2453T=KCTC 82653 T）、弯曲Undibacterium curvumsp. nov.（模式菌株CY 22 WT =GDMCC 1.1906T=KACC 21951 T）、芬田Undibacterium fentianensesp. nov.（模式菌株FT 137 WT =GDMCC 1.2456 T =KCTC 82656 T）、黄色Undibacterium flavidumsp. nov.（模式菌株LX 15 WT =GDMCC 1.1910 T =JCM 34286 T）、灰色Undibacterium griseumsp. nov.（模式菌株FT 31 WT =GDMCC 1.1908 T =KACC 21953 T）、Undibacterium hunanensesp. nov.（模式菌株CY 18 WT =GDMCC 1.1904T=KACC 21949 T）、罗富Undibacterium luofuensesp. nov.（模式菌株LFS 511 WT =GDMCC 1.2458T=KCTC 82658 T）、光亮Undibacterium nitidumsp. nov.（模式菌株LX 22 WT =GDMCC 1.1912T=KACC 21957 T）、里维斯Undibacterium rivisp. nov.（模式菌株FT 147 WT =GDMCC 1.2457 T =KCTC 82657 T）、皱纹Undibacterium rugosumsp. nov.（模式菌株CY 7 WT =GDMCC 1.1903T=KACC 21961 T）和伞形Undibacterium umbellatum sp. nov.（模式菌株NL 8 WT =GDMCC 1.1915T=KACC 21960 T）。
Twelve Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile strains (CY7WT, CY18WT, CY22WT, FT31WT, FT137WT, FT147WT, BYS50W, BYS107WT, LFS511WT, LX15WT, LX22WTand NL8WT) were isolated from streams in China. Comparisons based on 16S rRNA gene sequences indicated that these strains take species of genusUndibacteriumas close neighbours. The reconstructed phylogenetic and phylogenomic trees also showed that these strains cluster with species of genusUndibacteriumtogether. The genome G+C contents of these strains were in the range of 45.3 to 53.3 mol%. The calculated pairwise OrthoANIu values and digital DNA–DNA hybridization values among these strains and related strains were in the range of 70.4 to 94.1% and 19.3 to 55.3% except that the values between strains CY7WTand BYS50W were 99.0 and 91.8 %, respectively. Q-8 was their predominant respiratory quinone. C16 : 1ω7c and C16 : 0were their major fatty acids. Their polar lipids profiles were similar, including phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and two kinds of unidentified aminolipids. Combining polyphasic taxonomic characteristics and phylogenetic relationships, twelve strains should represent eleven independent novel species of genusUndibacterium, for which the namesUndibacterium baiyunensesp. nov. (type strain BYS107WT=GDMCC 1.2453T=KCTC 82653T),Undibacterium curvumsp. nov. (type strain CY22WT=GDMCC 1.1906T=KACC 21951T),Undibacterium fentianensesp. nov. (type strain FT137WT=GDMCC 1.2456T=KCTC 82656T),Undibacterium flavidumsp. nov. (type strain LX15WT=GDMCC 1.1910T=JCM 34286T),Undibacterium griseumsp. nov. (type strain FT31WT=GDMCC 1.1908T=KACC 21953T),Undibacterium hunanensesp. nov. (type strain CY18WT=GDMCC 1.1904T=KACC 21949T),Undibacterium luofuensesp. nov. (type strain LFS511WT=GDMCC 1.2458T=KCTC 82658T),Undibacterium nitidumsp. nov. (type strain LX22WT=GDMCC 1.1912T=KACC 21957T),Undibacterium rivisp. nov. (type strain FT147WT=GDMCC 1.2457T=KCTC 82657T),Undibacterium rugosumsp. nov. (type strain CY7WT=GDMCC 1.1903T=KACC 21961T) andUndibacterium umbellatumsp. nov. (type strain NL8WT=GDMCC 1.1915T=KACC 21960T) are proposed.