A label-free and self-assembled electrochemical biosensor for highly sensitive detection of cyclic diguanylate monophosphate (c-di-GMP) based on RNA riboswitch.
A label-free and self-assembled electrochemical biosensor for highly sensitive detection of cyclic diguanylate monophosphate (c-di-GMP) based on RNA riboswitch.
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DOI：
10.1016/j.aca.2015.04.061
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发表时间：
2015-07
影响因子：
6.2
通讯作者：
Qingyun Xie;Fulin Zhao;Hongrui Liu;Yanke Shan;Fei Liu
中科院分区：
文献类型：
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作者：
Qingyun Xie;Fulin Zhao;Hongrui Liu;Yanke Shan;Fei Liu
环状二鸟苷一磷酸(c-di-GMP)是一种重要的第二信使，调节多种细菌的运动、毒力、生物膜形成和细胞周期进程等多种复杂的生理过程。在这里，我们报道了一种简单的无标记和自组装的基于RNA核糖开关的生物传感器，用于灵敏和选择性地检测c-di-GMP。C-di-GMP的检测浓度范围为50 nm~1μM，检出限为50 nm。
Cyclic diguanylate monophosphate (c-di-GMP) is an important second messenger that regulates a variety of complex physiological processes involved in motility, virulence, biofilm formation and cell cycle progression in several bacteria. Herein we report a simple label-free and self-assembled RNA riboswitch-based biosensor for sensitive and selective detection of c-di-GMP. The detectable concentration range of c-di-GMP is from 50 nM to 1 μM with a detection limit of 50 nM.