Complementation of the Magnaporthe grisea deltacpkA mutation by the Blumeria graminis PKA-c gene: functional genetic analysis of an obligate plant pathogen.
Complementation of the Magnaporthe grisea deltacpkA mutation by the Blumeria graminis PKA-c gene: functional genetic analysis of an obligate plant pathogen.
复制标题
小麦白粉菌 PKA-c 基因对稻瘟病菌 deltacpkA 突变的补充：专性植物病原体的功能遗传分析。
DOI：
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发表时间：
2001
影响因子：
3.5
通讯作者：
R. Oliver
中科院分区：
文献类型：
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作者：
L. Bindslev;M. J. Kershaw;N. Talbot;R. Oliver
专性植物病原真菌已被证明非常难以用分子遗传学来表征，因为它们不能远离宿主植物培养，并且只能在有限的情况下进行实验操作。以前，为了表征白粉病真菌Blumeria graminis（syn.小麦白粉病菌（Erysiphe graminis）f. sp. hordei，我们描述了一个类似于环AMP依赖性蛋白激酶A的催化亚基的基因（这里更名为Bka 1）。通过在非专性病原体稻瘟病菌的deltacpkA突变体中表达，已经实现了该基因的功能表征。这种非致病性M. grisea deltacpkA突变体显示延迟和不完全的附着胞发育，表明PKA-c在控制感染细胞成熟的信号转导过程中的作用。用霉菌Bka 1开放阅读框架转化deltacpkA突变体，由M.稻瘟病菌MPG 1启动子，恢复致病性和附着胞成熟动力学。据我们所知，这些结果提供了专性病原体致病性的第一个功能遗传分析，并突出了调节致病真菌感染相关发育的信号成分的显着保守性。
Obligate plant-pathogenic fungi have proved extremely difficult to characterize with molecular genetics because they cannot be cultured away from host plants and only can be manipulated experimentally in limited circumstances. Previously, in order to characterize signal transduction processes during infection-related development of the powdery mildew fungus Blumeria graminis (syn. Erysiphe graminis) f. sp. hordei, we described a gene similar to the catalytic subunit of cyclic AMP-dependent protein kinase A (here renamed Bka1). Functional characterization of this gene has been achieved by expression in a deltacpkA mutant of the nonobligate pathogen Magnaporthe grisea. This nonpathogenic M. grisea deltacpkA mutant displays delayed and incomplete appressorium development, suggesting a role for PKA-c in the signal transduction processes that control the maturation of infection cells. Transformation of the deltacpkA mutant with the mildew Bka1 open reading frame, controlled by the M. grisea MPG1 promoter, restored pathogenicity and appressorium maturation kinetics. The results provide, to our knowledge, the first functional genetic analysis of pathogenicity in an obligate pathogen and highlight the remarkable conservation of signaling components regulating infection-related development in pathogenic fungi.