Monitoring the reactive oxygen species in spermatozoa during liquid storage of boar semen and its correlation with sperm motility, free thiol content and seasonality
Monitoring the reactive oxygen species in spermatozoa during liquid storage of boar semen and its correlation with sperm motility, free thiol content and seasonality
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DOI：
10.1111/and.14237
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发表时间：
2021-09-06
期刊：
影响因子：
2.4
通讯作者：
Kohsaka, Tetsuya
中科院分区：
文献类型：
--
作者：
Hoang Xuan Khoi;Shimizu, Kenji;Kohsaka, Tetsuya
氧化应激是影响精液质量的一个重要因素，但是，活性氧（ROS），提供直接洞察氧化状态的监测尚未尝试。本研究旨在监测猪精液液态保存过程中精子活性氧的变化，以确定其与精子活力、游离巯基（SH）含量及季节性的相关性。在秋季和春季采集杜洛克成年公猪的精液，用桑葚III稀释液稀释，15 ℃保存，每天检测精子活性氧（ROS）水平、巯基（SH）含量和活力。在秋季和春季期间制备的精子中的ROS水平一直很低，直到第4天和第5天的存储，分别，此后逐渐增加与精子活力的损失。精子活性氧水平的增加与较高的巯基水平和较低的活力，这是从第4天的存储和9月，或初秋较高的加重。这项研究表明，在液体储存过程中增加的精子ROS水平导致氧化损伤，导致精子活力丧失，可能是通过降低精子活力，这表明精子ROS监测有效地评估了公猪精液的质量。
Oxidative stress is an important factor affecting the quality of spermatozoa during liquid storage of boar semen; however, monitoring of reactive oxygen species (ROS) that provides direct insight into the oxidative status is not yet attempted. This study aimed to monitor ROS in boar sperm during liquid semen storage to determine its correlation with sperm motility and free thiol (SH) content, and seasonality. Ejaculate was collected from mature Duroc boars in a commercial farm in autumn and spring, diluted in Mulberry III extender, stored at 15 degrees C, and examined daily for sperm ROS level, SH content and motility. The ROS levels in spermatozoa prepared during autumn and spring were constantly low until days 4 and 5 of storage, respectively, which thereafter progressively increased in association with the loss of sperm motility. The increased sperm ROS level correlated with the higher SH level and lower motility, which was accentuated from day 4 of storage and was higher in September, or early autumn. This study indicates that increased sperm ROS levels during liquid storage results in oxidative damage, causing loss of sperm motility, presumably through decreased sperm viability, suggesting that sperm ROS monitoring effectively evaluates the quality of boar semen.