Dietary restriction reduces hippocampal neurogenesis and granule cell neuron density without affecting the density of mossy fibers.
Dietary restriction reduces hippocampal neurogenesis and granule cell neuron density without affecting the density of mossy fibers.
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DOI：
10.1016/j.brainres.2017.02.028
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发表时间：
2017-05-15
期刊：
影响因子：
2.9
通讯作者：
Mandyam CD
中科院分区：
文献类型：
--
作者：
Staples MC;Fannon MJ;Mysore KK;Dutta RR;Ongjoco AT;Quach LW;Kharidia KM;Somkuwar SS;Mandyam CD
海马结构在长时间的热量和饮食限制（DR）后发生显著的形态和功能变化。在这项研究中，我们测试是否延长DR的结果在海马神经发生，颗粒细胞神经元和苔藓纤维，所有这些都支持可塑性的齿状回的密度有害的改变。年轻成年动物经历自由进食（对照条件）或每隔一天喂食方案（DR条件）3个月。定量背侧和腹侧齿状回中Ki-67细胞和28天龄的5-溴-2 '-脱氧尿苷（BrdU）细胞的数量，以确定DR对齿状回解剖学定义区域中神经祖细胞的细胞增殖和存活的影响。定量颗粒细胞神经元和突触孔蛋白的密度，以确定DR对齿状回颗粒细胞神经元和苔藓纤维投射的影响。我们的研究结果表明，DR增加细胞增殖，同时减少腹侧齿状回新生神经元的存活，而不影响背侧齿状回的细胞数量。DR使背侧齿状回颗粒细胞神经元密度降低。颗粒细胞神经元数量的这些改变并不影响DR动物的苔藓纤维密度，这被视为突触孔蛋白表达没有差异。我们的研究结果表明，在齿状回的颗粒细胞神经元是容易受到慢性DR和齿状回亚区的颗粒细胞的重组是不产生伴随的改变，在齿状回神经元回路与这种类型的饮食限制。
The hippocampal formation undergoes significant morphological and functional changes after prolonged caloric and dietary restriction (DR). In this study we tested whether prolonged DR results in deleterious alterations in hippocampal neurogenesis, density of granule cell neurons and mossy fibers, all of which support plasticity in the dentate gyrus. Young adult animals either experienced free access to food (control condition), or every-other-day feeding regimen (DR condition) for 3 months. The number of Ki-67 cells and 28-day old 5-bromo-2’-deoxyuridine (BrdU) cells were quantified in the dorsal and ventral dentate gyrus to determine the effect of DR on cellular proliferation and survival of neural progenitor cells in the anatomically defined regions of the dentate gyrus. The density of granule cell neurons and synaptoporin were also quantified to determine the effect of DR on granule cell neurons and mossy fiber projections in the dentate gyrus. Our results show that DR increases cellular proliferation and concurrently reduces survival of newly born neurons in the ventral dentate gyrus without effecting the number of cells in the dorsal dentate gyrus. DR reduced density of granule cell neurons in the dorsal dentate gyrus. These alterations in the number of granule cell neurons did not affect mossy fiber density in DR animals, which was visualized as no differences in synaptoporin expression. Our findings demonstrate that granule cell neurons in the dentate gyrus are vulnerable to chronic DR and that the reorganization of granule cells in the dentate gyrus subregions is not producing concomitant alterations in dentate gyrus neuronal circuitry with this type of dietary restriction.