Enhancing sensitivity and selectivity in a label-free colorimetric sensor for detection of iron(II) ions with luminescent molybdenum disulfide nanosheet-based peroxidase mimetics.
Enhancing sensitivity and selectivity in a label-free colorimetric sensor for detection of iron(II) ions with luminescent molybdenum disulfide nanosheet-based peroxidase mimetics.
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DOI：
10.1016/j.bios.2016.01.037
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发表时间：
2016-06
影响因子：
12.6
通讯作者：
Yong Wang;Jie Hu;Qianfen Zhuang;Y. Ni
中科院分区：
文献类型：
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作者：
Yong Wang;Jie Hu;Qianfen Zhuang;Y. Ni
在本研究中，我们证明以钼酸钠和硫脲为前体水热法制备的发光二硫化钼（MoS2）纳米片具有类似过氧化物酶的活性，并且可以在过氧化氢（H2O2）存在下催化过氧化物酶底物邻苯二胺（OPD）氧化产生黄色。 反应。进一步将 Fe2+ 添加到纳米片中导致过氧化物酶模拟物的催化活性大大增强。利用该观察结果开发了一种用于检测 Fe2+ 的无标记比色纳米酶传感器。制造的 MoS2/OPD/H2O2 传感器表现出 0.01–0.8 µM 的宽线性范围，检测限为 7 nM。此外，发现与OPD/H2O2传感器相比，MoS2/OPD/H2O2传感器对Fe2+表现出更高的灵敏度和选择性，这表明MoS2纳米片可以提高Fe2+传感器的性能。进一步应用先进的化学计量学算法，即通过交替最小二乘法进行多元曲线分辨率 (MCR-ALS)，来解释 MoS2 纳米片提高 Fe2+ 传感器灵敏度和选择性的根源。收集所研究系统的时间依赖性紫外可见光谱数据，并将其提交给 MCR-ALS。结果表明，MoS2/OPD/H2O2传感器对Fe2+检测的灵敏度和选择性的提高可能是由于其较大的反应速率常数。最后，所提出的MoS2/OPD/H2O2传感器成功应用于水样中Fe2+的检测。
In the present study, we demonstrated that the luminescent molybdenum disulfide (MoS2) nanosheets, which were prepared hydrothermally by using sodium molybdate and thiourea as precursors, possessed peroxidase-like activity, and could catalyze the oxidation of peroxidase substrate o-phenylenediamine (OPD) in the presence of hydrogen peroxide (H2O2) to produce a yellow color reaction. Further addition of Fe2+into the nanosheets led to peroxidase mimetics with greatly enhanced catalytic activity. The observation was exploited to develop a label-free colorimetric nanozyme sensor for detection of Fe2+. The fabricated MoS2/OPD/H2O2sensor showed a wide linear range of 0.01–0.8 µM with a detection limit of 7 nM. Moreover, it was found that the MoS2/OPD/H2O2sensor displayed enhanced sensitivity and selectivity toward Fe2+compared with the OPD/H2O2sensor, suggesting that the MoS2nanosheets could improve the performance of the Fe2+sensor. An advanced chemometrics algorithm, multivariate curve resolution by alternating least squares (MCR-ALS), was further applied to interpret the origin of enhancing sensitivity and selectivity in the Fe2+sensor with the MoS2nanosheets. The time-dependent UV–vis spectral data of the studied systems were collected, and submitted to the MCR-ALS. The results showed that the increased sensitivity and selectivity of the MoS2/OPD/H2O2sensor for Fe2+detection likely arose from its large reaction rate constant. Finally, the proposed MoS2/OPD/H2O2sensor was successfully applied for detection of Fe2+in water samples.