Comparative analysis of intestinal microbial community diversity between healthy and orally infected ducklings with Salmonella enteritidis by ERIC-PCR
Comparative analysis of intestinal microbial community diversity between healthy and orally infected ducklings with Salmonella enteritidis by ERIC-PCR
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DOI：
10.3748/wjg.14.1120
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发表时间：
2008-02-21
影响因子：
4.3
通讯作者：
Chen, Xiao-Yue
中科院分区：
文献类型：
--
作者：
Cao, Sheng-Yan;Wang, Ming-Shu;Chen, Xiao-Yue
目的：分析健康雏鸭与口腔感染肠炎沙门氏菌雏鸭肠道微生物群落多样性的差异。方法：采用肠杆菌重复基因间一致性(ERIC)-PCR方法，分析健康雏鸭和7日龄雏鸭口腔感染肠炎沙门氏菌后不同时间点十二指肠、空肠、回肠、盲肠、直肠等肠道微生物群落多样性及动态变化。结果：对照组健康雏鸭肠道微生物群落稳定，直肠组ERIC-PCR条带数最少，盲肠组最多。经口接种的雏鸭ERIC-PCR条带直到接种后24 h （p.i）才发生明显变化。从24 h到72 h， ERIC-PCR条带数量逐渐减少，随着病情的发展，条带数量逐渐增加，直到6 d。主要细菌因肠炎沙门氏菌感染而改变，ERIC-PCR主片段DNAstar显示，需氧菌和兼性需氧菌（大肠杆菌、志贺氏菌、沙门氏菌）成为口腔感染SE雏鸭肠道的优势菌群。结论：本研究为阐明健康雏鸭和口腔感染肠炎沙门氏菌雏鸭肠道微生物群落多样性和动态变化提供了重要数据，为人类和动物肠炎沙门氏菌感染的发病机制提供了有价值的见解。(c) 2008 WJG。版权所有。
AIM: To analyze the difference of intestinal microbial community diversity between healthy and (S enteritidis) orally infected ducklings.METHODS: Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR was applied to analyze the intestinal microbial community diversity and dynamic change including duodenum, jejunum, ileum, cecum and rectum from healthy ducklings and 7-day-old ducklings after oral infection with S. enteritidis at different time points.RESULTS: The intestinal microbial community of the control healthy ducklings was steady and the ERIC-PCR band numbers of the control healthy ducklings were the least with rectum and were the most with caecum. ERIC-PCR bands of orally inoculated ducklings did not obviously change until 24 h after inoculation (p.i.). The numbers of the ERIC-PCR bands gradually decreased from 24 h to 72 h p.i., and then, with the development of disease, the band numbers gradually increased until 6 d p.i. The prominent bacteria changed because of S. enteritidis infection and the DNAstar of staple of ERIC-PCR showed that aerobe and facultative aerobe (Escherichia coli, Shigella, Salmonella) became preponderant bacilli in the intestine of orally infected ducklings with SE.CONCLUSION: This study has provided significant data to clarify the intestinal microbial community diversity and dynamic change of healthy and S. enteritidis orally infected ducklings, and valuable insight into the pathogenesis of S. enteritidis infection in both human and animals. (c) 2008 WJG. All rights reserved.