METABOLISM OF CONSTITUENT LIPIDS OF DOG CHYLOMICRONS.
METABOLISM OF CONSTITUENT LIPIDS OF DOG CHYLOMICRONS.
复制标题
狗乳糜微粒的脂质成分的代谢。
DOI：
10.1172/jci104815
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发表时间：
1963
期刊：
影响因子：
--
通讯作者：
A. Bezman
中科院分区：
文献类型：
--
作者：
P. Nestel;R. Havel;A. Bezman
方法标记乳糜微粒的制备。对1、2、4和5号狗的实验中，通过给供体狗喂500 μ c的棕榈酸钠-9，10-HW和50 μ c的胆固醇-4-C1 ′，标记乳糜微粒中的TGFA、酯胆固醇和游离胆固醇。在其他实验中，在喂食其他同位素前4小时，给供体狗静脉注射5 μ g磷酸钠Pa，标记乳糜微粒中的磷脂。通过插入胸导管的插管从供体犬获得淋巴液。通过在0.15M NaCl下分层淋巴来分离乳糜微粒。在Spinco型号L超离心器的30转子中以20，000 rpm离心30分钟后，通过切管器分离上清液。收集乳糜微粒并重悬于盐水中。
METHODSPreparation of labeled chylomicrons. For the experi-ments ondogs 1, 2, 4, and 5, TGFA, ester cholesterol, and free cholesterol in the chylomicrons were labeled by feeding donor dogs 500, uc of sodium palmitate-9, 10-HW and 50 uc of cholesterol-4-C1'with milk. For the other experiments, the phospholopids in the chylomicrons were also labeled by iv injection of 5 mc of sodium phosphate Pa into donor dogs 4 hours before feeding theother isotopes. Lymph was obtained from the donor dogs through a cannula inserted in the thoracic duct. The chylomi-crons were separated bylayering the lymph under 0.15 M NaCI. After centrifugation for 30 minutes at 20,000 rpm in the 30 rotor of a Spinco model L ultracentrifuge, the supernatant fluid was separated by means of a tube slicer. The chylomicrons were collected and resuspended in saline.