Effect of fructose on the phosphorylation of AMP-activated protein kinase and acetyl-CoA carboxylase in HepG2 cells stimulated with placental lactogen.
Effect of fructose on the phosphorylation of AMP-activated protein kinase and acetyl-CoA carboxylase in HepG2 cells stimulated with placental lactogen.
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DOI：
10.1002/bdrb.21186
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发表时间：
2016-08
期刊：
影响因子：
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通讯作者：
Y. Mukai;Fumika Hoshi;Shin Sato
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文献类型：
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作者：
Y. Mukai;Fumika Hoshi;Shin Sato
高果糖摄入通过肝脏和外周组织中的AMP活化蛋白激酶（AMPK）信号传导诱导脂质代谢的破坏。母体脂质代谢在生理上被妊娠激素如人胎盘催乳素（PL）的活性改变。为了阐明妊娠期间高果糖摄入对肝脏脂质代谢的影响，我们研究了果糖通过PL刺激肝细胞中AMPK途径对脂质代谢的影响。方法用D（-）-果糖处理人肝癌细胞（HepG 2），加入或不加入PL。测定细胞内脂质含量。通过Western印迹法定量AMPK和乙酰辅酶A羧化酶（ACC）的总蛋白和磷酸化蛋白含量。结果与未处理的细胞相比，PL处理的HepG 2细胞内三酰甘油水平显著降低，但PL不影响细胞内三酰甘油水平。在PL存在下，果糖处理的HepG 2细胞中AMPK和ACC磷酸化显著增加，并呈浓度依赖性。结论果糖通过AMPK/ACC信号途径降低PL刺激的肝细胞中三酰甘油水平。这些发现表明，妊娠期高果糖摄入可能会损害母体肝脏的脂质代谢。
BACKGROUND High fructose intake induces disruption of lipid metabolism via AMP-activated protein kinase (AMPK) signaling in the liver and peripheral tissues. Maternal lipid metabolism is physiologically altered by the activity of pregnancy hormones such as human placental lactogen (PL). To elucidate the influence of high fructose intake on hepatic lipid metabolism during pregnancy, we examined the effects of fructose on lipid metabolism via the AMPK pathway in hepatocytes stimulated with PL. METHODS Human hepatoma cells (HepG2) were treated with D(-)-fructose in the presence or absence of PL. Intracellular lipid contents were measured. The total and phosphorylated protein content of AMPK and acetyl-CoA carboxylase (ACC) was quantified by Western blotting. RESULTS The intracellular triacylglycerol level in fructose-treated HepG2 cells decreased significantly compared with that in untreated cells in the presence, but not absence, of PL. AMPK and ACC phosphorylation increased significantly and concentration-dependently in fructose-treated HepG2 cells in the presence of PL. CONCLUSION Our results suggest that fructose treatment reduces triacylglycerol levels via AMPK/ACC signaling in PL-stimulated hepatocytes. These findings suggest that high fructose intake during pregnancy might impair lipid metabolism in the maternal liver.