Within brown-fat cells, UCP1-mediated fatty acid-induced uncoupling is independent of fatty acid metabolism
Within brown-fat cells, UCP1-mediated fatty acid-induced uncoupling is independent of fatty acid metabolism
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DOI：
10.1016/j.bbabio.2008.04.038
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发表时间：
2008-07-01
影响因子：
4.3
通讯作者：
Nedergaard, Jan
中科院分区：
文献类型：
--
作者：
Shabalina, Irina G.;Backlund, Emma C.;Nedergaard, Jan
In the present investigation, we have utilized the availability of UCP1(-/-) mice to examine a wide range of previously proposed lipid activators of Uncoupling Protein 1 (UCP1) in its native environment, i.e. in the brown-fat cells. A non-metabolizable fatty acid analogue, beta,beta c-methyl-substituted hexadecane alpha,omega-dicarboxylic acid (Medica-16) is a potent UCP1 (re)activator in brown-fat cells, despite its bipolar structure. All-trans-retinoic acid activates UCP1 within cells, whereas beta-carotene only does so after metabolism. The UCP1-dependent effects of fatty acids are positively correlated with their chain length. Medium-chain fatty acids are potent UCP1 activators in cells, despite their lack of protonophoric properties in mitochondrial membranes. Thus, neither the ability to be metabolized nor an innate uncoupling/protonophoric ability is a necessary property of UCP1 activators within brown-fat cells. (c) 2008 Elsevier B.V. All rights reserved.
