Extraction and Analysis of Tomato Seed Oil
Extraction and Analysis of Tomato Seed Oil
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DOI：
10.1007/s11746-010-1563-4
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发表时间：
2010-07-01
影响因子：
2
通讯作者：
Taylor, S. L.
中科院分区：
文献类型：
--
作者：
Eller, F. J.;Moser, J. K.;Taylor, S. L.
番茄籽是将西红柿加工成番茄汁、酱油和酱油等产品过程中产生的一种非常大的副产品。这些种子的一个潜在用途是作为植物油的来源。本研究采用多种提取技术对番茄种子的含油量进行了研究，并对番茄种子的油脂提取物进行了检测，以确定番茄种子中植物甾醇和抗氧化剂等次要成分的组成。测定了番茄籽油的氧自由基吸收能力(ORAC)，并与抗氧化剂含量进行了相关分析。本研究表明，用热乙醇提取番茄籽油的得油率最高，其次是热正己烷，最后是超临界CO2。然而，SC-CO2处理具有最高的总植物甾醇含量和最高的单株植物甾醇含量。谷甾醇、环丙醇和豆甾醇是提取物中含量最丰富的植物甾醇。抗氧化剂在正己烷提取物中的浓度最高。在番茄籽油中发现的最丰富的抗氧化剂是全反式番茄红素、顺式-3-番茄红素和β-胡萝卜素。正己烷提取物的ORAC含量最高。产油量与α-生育酚、γ-生育酚含量呈负相关，与顺式-3-番茄红素含量呈正相关。ORAC值仅与全反式番茄红素和顺式-3-番茄红素呈正相关，表明它们在番茄籽油中具有抗氧化剂的作用。
Tomato seeds represent a very large waste by-product from the processing of tomatoes into products such as tomato juice, sauce and paste. One potential use for these seeds is as a source of vegetable oil. This research investigated the oil content of tomato seeds using several extraction techniques as well as an examination of the oil extracts to determine the composition of the minor constituents such as phytosterol and antioxidant composition. The oxygen radical absorbance capacity (ORAC) of the tomato seed oils were also measured and correlated with antioxidant contents. This research demonstrated that tomato seed oil yield was highest using hot ethanol and followed by hot hexane and finally SC-CO2. The SC-CO2 treatment, however, had the highest total phytosterol content as well as highest individual phytosterol content. Sitosterol, cycloartanol, and stigmasterol were the most abundant phytosterols present in the extracts. The highest concentrations of antioxidants were found in the hexane extract. The most abundant antioxidants found in the tomato seed oils were all-trans-lycopene, cis-3-lycopene and beta-carotene. ORAC was highest for the hexane extract. Oil yield was inversely proportional to both alpha-tocopherol and gamma-tocopherol content and positively correlated with cis-3-lycopene content. ORAC values were positively correlated with only all-trans-lycopene and cis-3-lycopene demonstrating their role as antioxidants in the tomato seed oil.