Apoptosis: a method for evaluating the cryopreservation of whole blood and peripheral blood mononuclear cells
Apoptosis: a method for evaluating the cryopreservation of whole blood and peripheral blood mononuclear cells
复制标题
DOI：
10.1016/s0022-1759(00)00263-5
复制
发表时间：
2000-10-20
影响因子：
2.2
通讯作者：
Cosentino, M
中科院分区：
文献类型：
--
作者：
Fowke, KR;Behnke, J;Cosentino, M
我们试图比较人全血与匹配的外周血单核细胞（PBMC）样本在-30度、-70度和- 150度低温保存和储存的效果，并将细胞凋亡作为细胞适应性的指标。保存10周后，将样品解冻并评估活力（台盼蓝排除）、细胞凋亡水平（使用核染色双苯并胺）和细胞功能（eb病毒转化能力）。当比较储存温度时，-30℃保存的全血和PBMC样品的细胞凋亡水平明显高于-70℃或- 150℃保存的样品(P
We sought to compare the effect of cryopreservation and storage at -30 degreesC, -70 degreesC and - 150 degreesC of human whole blood versus matched peripheral blood mononuclear cell (PBMC) samples using apoptosis as an indicator of cell fitness. Following 10 weeks of storage the samples were thawed and assessed for viability (trypan blue exclusion), levels of apoptosis (using the nuclear stain bis-benzimide) and cell function (ability to be transformed by Epstein-Barr virus, EBV). When comparing storage temperatures, the Levels of apoptosis in whole blood and PBMC samples stored at -30 degreesC were significantly higher than the values for samples stored at -70 degreesC or - 150 degreesC (P