Cadmium exposure promotes activation of cerebrum and cerebellum ferroptosis and necrosis in swine
Cadmium exposure promotes activation of cerebrum and cerebellum ferroptosis and necrosis in swine
复制标题
镉暴露促进猪大脑和小脑铁死亡和坏死的激活
DOI：
10.1016/j.ecoenv.2021.112650
复制
发表时间：
2021
影响因子：
6.8
通讯作者：
Zhang Ziwei
中科院分区：
文献类型：
--
作者：
Luan Peixian;Sun Yue;Zhu Yue;Qiao Senqiu;Hu Guo;Liu Qi;Zhang Ziwei
Cadmium(Cd) is a toxic and carcinogenic heavy metal pollutant leading to serious damage in various organs. Ferroptosis and necrosis as inflammation-related cell death are involved in several diseases of nervous system. In the present study, 10 weaning piglets with similar weight for 6 weeks were randomly divided into two groups. The daily grain containing 0 mg and 20 mg/kg of Cd chloride was fed in 20–26 ℃ environment, animals were sacrificed to collect cerebrum and cerebellum tissues after 40 days. Morphology and ultrastructure results were observed using HE and TEM. Moreover, molecular biological technologies western blot and qRT-PCR were used to detect the expression abundance of genes. Cerebrum and cerebellum injury was observed in Cd-exposed group, antioxidant capacity decreased significantly and oxidative stress increased; immunofluorescence, real-time quantification, and western blot results showed decreased necrosis genes and increased ferroptosis pathway genes abundance in cerebrum, whereas the results were reversed in cerebellum. These results indicated that Cd exposure can activated necrosis and ferroptosis pathways by increased oxidative stress, further resulting in cerebrum and cerebellum damage in pigs. These findings may provide a theoretical basis for early monitoring of Cd exposure in environment.
