Contribution of Drosophila DEG/ENaC genes to salt taste
Contribution of Drosophila DEG/ENaC genes to salt taste
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DOI：
10.1016/s0896-6273(03)00394-5
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发表时间：
2003-07-03
期刊：
影响因子：
16.2
通讯作者：
Welsh, MJ
中科院分区：
文献类型：
--
作者：
Liu, L;Leonard, AS;Welsh, MJ
探测盐的能力对于陆生动物的生存至关重要。基于阿米洛利依赖性抑制，检测盐的受体被假定为DEG/ENaC通道。我们发现果蝇DEG/ENaC基因Pickpocket 11（ppk 11）和Pickpocket 19（ppk 19）在幼虫的味觉感受终末器官和成虫的唇瓣、腿和翅缘的味觉刚毛中表达。当我们破坏PPK 11或PPK 19功能时，幼虫失去了区分低浓度Na+或K+与水的能力，对低盐浓度的电生理反应减弱。在幼虫和成虫中，破坏PPK 11或PPK 19会影响对高盐浓度的行为反应。与此相反，幼虫蔗糖，pH值3，和几种气味的反应保持完整。这些结果表明DEG/ENaC通道PPK 11和PPK 19在检测Na+和K+盐中起关键作用。
The ability to detect salt is critical for the survival of terrestrial animals. Based on amiloride-dependent inhibition, the receptors that detect salt have been postulated to be DEG/ENaC channels. We found the Drosophila DEG/ENaC genes Pickpocket11 (ppk11) and Pickpocket19 (ppk19) expressed in the larval taste-sensing terminal organ and in adults on the taste bristles of the labelum, the legs, and the wing margins' When we disrupted PPK11 or PPK19 function, larvae lost their ability to discriminate low concentrations of Na+ or K+ from water, and the electrophysiologic responses to low salt concentrations were attenuated. In both larvae and adults, disrupting PPK11 or PPK19 affected the behavioral response to high salt concentrations. In contrast, the response of larvae to sucrose, pH 3, and several odors remained intact. These results indicate that the DEG/ENaC channels PPK11 and PPK19 play a key role in detecting Na+ and K+ salts.