Surface plasmon resonance scattering and absorption sensing of Concanavalin A using glycoconjugated gold nanoparticles
Surface plasmon resonance scattering and absorption sensing of Concanavalin A using glycoconjugated gold nanoparticles
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DOI：
10.1080/10610278.2010.527977
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发表时间：
2011-03
影响因子：
3.3
通讯作者：
Shigeru Watanabe;S. Yamamoto;Kazuma Yoshida;K. Shinkawa;Daisuke Kumagawa;Hideki Seguchi
中科院分区：
文献类型：
--
作者：
Shigeru Watanabe;S. Yamamoto;Kazuma Yoshida;K. Shinkawa;Daisuke Kumagawa;Hideki Seguchi
糖基金纳米粒子（AuNPs）作为检测碳水化合物-蛋白质相互作用的探针受到极大关注。三种传感方法-吸收，光散射和波长比散射-用于检测甘露糖缀合的金纳米颗粒和伴刀豆球蛋白A（Con A）之间的多价相互作用。蛋白质-碳水化合物相互作用诱导纳米颗粒聚集，使等离子体吸收向更长波长移动，并增加等离子体光散射强度。等离子体吸收波长移动和散射光强度增强分别与Con A的浓度在5.7-20.2 nM和3.8-36.7 nM范围内成正比，相应的检测限分别为3.4和2.3 nM。波长比率散射法显示出最低的检测限（1.9 nM）的Con A，在5-40倍低于使用现有的糖共轭金纳米粒子。
Glycoconjugated gold nanoparticles (AuNPs) are of great interest as probes for detecting carbohydrate–protein interactions. Three sensing methods – absorption, light scattering and wavelength-ratiometric scattering – were used to detect polyvalent interactions between mannose-conjugated AuNPs and Concanavalin A (Con A). Nanoparticle aggregation was induced by protein–carbohydrate interaction, which shifted the plasmon absorption to longer wavelengths and increased the intensity of plasmon light scattering. The plasmon absorption wavelength shift and scattering light intensity enhancement were proportional to the concentration of Con A in the range 5.7–20.2 nM and 3.8–36.7 nM, respectively, and the corresponding limits of detection were 3.4 and 2.3 nM, respectively. The wavelength-ratiometric scattering method showed the lowest detection limits (1.9 nM) for Con A, at 5–40 times lower than those obtained using existing glycoconjugated AuNPs.