A visible and colorimetric aptasensor based on DNA-capped single-walled carbon nanotubes for detection of exosomes
A visible and colorimetric aptasensor based on DNA-capped single-walled carbon nanotubes for detection of exosomes
复制标题
一种基于 DNA 封端单壁碳纳米管的可见比色适体传感器，用于检测外泌体
DOI：
10.1016/j.bios.2017.01.063
复制
发表时间：
2017-06-15
影响因子：
12.6
通讯作者：
Chen, Jinghua
中科院分区：
文献类型：
--
作者：
Xia, Yaokun;Liu, Mengmeng;Chen, Jinghua
最近，许多研究表明，循环外泌体作为新型生物标志物用于监测和预测包括癌症在内的许多复杂疾病的潜在用途。然而，在常规临床环境中可靠且具有成本效益的外泌体检测仍然是一项艰巨的任务，这主要是由于缺乏足够简单和快速的测定平台。因此，我们在这里展示了一个可见的和简单的方法检测外泌体的发展，通过整合单壁碳纳米管，是优秀的水溶性（s-SWCNTs）和适体。针对外泌体跨膜蛋白CD 63的适体被吸附到s-SWCNT表面，提高s-SWCNT 5的微量过氧化物酶活性，可以有效催化H2 O2介导的3，3 '，5，5'-四甲基联苯胺（TMB）氧化，导致溶液从无色变为蓝色。然而，加入外泌体后，适体与CD 63结合，通过构象变化从s-SWCNT表面离开，这导致溶液的颜色从深到中等，这可以通过肉眼观察并通过UV维斯光谱法监测。在最佳条件下，外泌体的线性范围估计为1.84x10（6）至2.21x10（7）个颗粒/μ L，检测限（LOD）为5.2x10（5）个颗粒/μ L。因此，成功构建了一种可见且简单的检测外泌体的方法。此外，这种比色适体传感器可以通过简单的改变适体而普遍适用于其他靶标的检测。
Recently, many studies have shown the potential use of circulating exosomes as novel biomarkers for monitoring and predicting a number of complex diseases, including cancer. However, reliable and cost-effective detection of exosomes in routine clinical settings, still remain a difficult task, mainly due to the lack of adequately easy and fast assay platforms. Therefore, we demonstrate here the development of a visible and simple method for the detection of exosomes by integrating single-walled carbon nanotubes that being excellent water solubility (s-SWCNTs) and aptamer. Aptamers, specific to exosomes transmembrane protein CD63, are absorbed onto the surface of s-SWCNTs and improve the minic peroxidase activity of s-SWCNT5, which can efficiently catalyze H2O2-mediated oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) and lead to a change from colorless to blue in solution. However, after adding exosomes, the aptamers are bound with CD63, leaving from the surface of s-SWCNTs through conformational changes, which results the color of solution from deep to moderate, and this can be observed by the naked eye and monitored by UV vis spectrometry. Under optimal conditions, the linear range of exosomes is estimated to be 1.84x10(6) to 2.21x10(7) particles/mu L with a detection of limit (LOD) of 5.2x10(5) particles/mu L. Consequently, a visible and simple approach detecting exosomes is successfully constructed. Moreover, this proposed colorimetric aptasensor can be universally applicable for the detection of other targets by simple change the aptamer.