Adeno-associated viral vectors for anterograde axonal tracing with fluorescent proteins in nontransgenic and cre driver mice.
Adeno-associated viral vectors for anterograde axonal tracing with fluorescent proteins in nontransgenic and cre driver mice.
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DOI：
10.1002/0471142301.ns0120s59
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发表时间：
2012-04-01
影响因子：
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通讯作者：
Zeng, Hongkui
中科院分区：
文献类型：
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作者：
Harris, Julie A;Oh, Seung Wook;Zeng, Hongkui
利用病毒感染有丝分裂后细胞（如神经元）的天然能力，提供了大量新方法来操纵和重建体内神经回路。在这里，我们描述了使用重组腺相关病毒载体（rAAV）的轴突束追踪在非转基因和转基因Cre驱动器小鼠。提出了两种方案，用于使用离子电渗或纳升压力注射将rAAV载体立体定向引导放置到活小鼠脑中。这里讨论的方法将导致荧光蛋白在目标神经元的细胞体、树突和轴突中表达，并且可以很容易地适应于解决各种实验问题。
Harnessing the natural ability of viruses to infect post-mitotic cells such as neurons has provided an explosion of new methods to manipulate and reconstruct neural circuits in vivo. Here we describe the use of recombinant adeno-associated viral vectors (rAAV) for axonal tract tracing in nontransgenic and transgenic Cre driver mice. Two protocols are presented for stereotactic-guided placement of rAAV vectors into the live mouse brain using iontophoretic or nanoliter pressure injections. The methods discussed here will result in expression of fluorescent proteins in cell bodies, dendrites, and axons in targeted neurons, and can be easily adapted to address various experimental questions.