Molecular analysis of Dec1 and Dec2 in the peripheral circadian clock of zebrafish photosensitive cells
Molecular analysis of Dec1 and Dec2 in the peripheral circadian clock of zebrafish photosensitive cells
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DOI：
10.1016/j.bbrc.2006.10.172
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发表时间：
2006-12-29
影响因子：
3.1
通讯作者：
Iigo, Masayuki
中科院分区：
文献类型：
--
作者：
Abe, Tomotaka;Ishikawa, Tomoko;Iigo, Masayuki
为了阐明基本螺旋-环-螺旋转录因子DEC1和DEC2在光敏斑马鱼外周细胞生物钟中的作用，克隆了斑马鱼DEC1和DEC2 (zDec1和zDec2)，并在斑马鱼BRF41细胞系中检测了它们的功能和表达模式。zDEC1和zDEC2序列与哺乳动物序列具有较高的相似性，zDEC1和zDEC2序列的分子系统发育分析反映了预测的物种分类模式。zDEC1和zDEC2抑制zCLOCK1:zBMAL3介导的CRY1a转录。zDec1和zDec2 mRNA在BRF41细胞中表现出强烈的昼夜振荡。zDec1和zDec2 mRNA受光照的影响不明显。这些结果表明，zDec1和zDec2通过抑制clock / bmal诱导的基因表达参与光敏斑马鱼外周细胞的蝉鸣时钟机制，并且zDec1和zDec2的反馈回路可能与PER/CRY核心昼夜反馈回路互锁。(c) 2006爱思唯尔公司版权所有。
To elucidate the roles of DEC1 and DEC2, basic helix-loop-helix transcription factors, in the circadian clock of photosensitive zebrafish peripheral cells, zebrafish Dec1 and Dec2 (zDec1 and zDec2) were cloned and their functions and expression patterns were examined in BRF41, a zebrafish cell line. zDEC1 and zDEC2 have high sequence similarity to mammalian counterparts and the molecular phylogenetic analysis of the zDEC1 and zDEC2 sequences reflected the predicted pattern of species classification. zDEC1 and zDEC2 inhibited zCLOCK1:zBMAL3 mediated transcription as CRY1a. zDec1 and zDec2 mRNA showed robust circadian oscillation in BRF41 cells. However, zDec1 and zDec2 mRNA was not strongly induced by exposure to light. These results indicate that zDec1 and zDec2 are involved in the cicadian clock mechanism in photosensitive zebrafish peripheral cells by suppressing CLOCK/BMAL-induced gene expression and that the feedback loops of zDEC1 and zDEC2 may be interlocked with the PER/CRY core circadian feedback loops. (c) 2006 Elsevier Inc. All rights reserved.