Virulence of Wolinella recta in a murine abscess model.
Virulence of Wolinella recta in a murine abscess model.
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鼠脓肿模型中直肠沃利氏菌的毒力。
DOI：
10.1128/iai.59.8.2806-2817.1991
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发表时间：
1991
影响因子：
3.1
通讯作者：
Ebersole,JL
中科院分区：
文献类型：
--
作者：
Kesavalu,L;Holt,SC;Crawley,RR;Borinski,R;Ebersole,JL
通过使用 BALB/c 小鼠的单一感染，在实验动物模型中研究了直肠沃利氏菌分离株的毒力。临床分离株W.直肠576和W.直肠234的感染引起干燥、平坦、凹陷的坏疽性坏死性皮肤损伤，而W.直肠ATCC 33238未能诱导类似的损伤。感染后72小时皮损组织学检查显示表皮、皮下组织和皮干肌凝固性坏死，血清蛋白和中性粒细胞明显渗出。毒力调节剂如地塞米松、半乳糖胺、硫酸肼和葡聚糖微载体珠与直肠蠕虫感染结合使用。与未治疗的对照小鼠相比，地塞米松、硫酸肼和葡聚糖珠增强了直肠蠕虫对病变形成和组织破坏的感染性和致病性。半乳糖胺致敏增强了直肠威氏菌的毒力潜力，达到了观察到致死结果的程度。临床分离株的实验室传代表明，高传代菌株的毒力降低，这与广泛传代的直肠壁球菌 ATCC 33238 中观察到的最低毒力相关。在感染动物的血清中检测到血清免疫球蛋白 G (IgG) 和 IgM 反应，交叉反应抗体表明各种直壁壁球菌菌株的抗原组成存在差异。第二次攻击后观察到 IgG 抗体反应增强。对初始感染产生获得性抗体反应的小鼠在随后的攻击中仍然容易形成病变，但病变的大小显着减小，表明有部分保护。与从感染中恢复的小鼠相比，主动免疫后血清 IgG 和 IgM 抗体水平显着升高。免疫接种显着减小了病灶大小；然而，即使如此高水平的抗体也未能消除损伤诱导。
The virulence of Wolinella recta isolates was studied in an experimental animal model by using monoinfection of BALB/c mice. Infection with clinical isolates of W. recta 576 and W. recta 234 induced dry, flat, depressed gangrenous necrotic skin lesions, whereas W. recta ATCC 33238 failed to induce a similar lesion. Histological examination of the skin lesion 72 h postinfection revealed coagulation necrosis of the epidermis, subcutis and cutaneous truncus muscle, with marked exudation of serum proteins and neutrophils. Virulence-modulating agents such as dexamethasone, galactosamine, hydrazine sulfate, and dextran microcarrier beads were used in conjunction with W. recta infection. Dexamethasone, hydrazine sulfate, and dextran beads enhanced the infectivity and pathogenicity of W. recta for lesion formation and tissue destruction compared with what was found in untreated control mice. Galactosamine sensitization enhanced the virulence potential of W. recta to such an extent that a lethal outcome was observed. Laboratory passage of clinical isolates demonstrated a decreased virulence in high-passage strains, which correlated with the minimal virulence observed in the extensively passaged W. recta ATCC 33238. Serum immunoglobulin G (IgG) and IgM responses were detected in the serum of infected animals, and cross-reacting antibody indicated variation in the antigenic makeup of various W. recta strains. Enhanced IgG antibody responses were observed following the secondary challenge. Mice with acquired antibody response to initial infection remained susceptible to lesion formation with subsequent challenge, but the size of the lesion was significantly reduced, indicating partial protection. Serum IgG and IgM antibody levels were significantly increased by active immunization when compared with levels in mice which had recovered from infection. The immunization significantly decreased the lesion size; however, even these high levels of antibody failed to abrogate the lesion induction.