Description of Naumannella cuiyingiana sp nov., isolated from a ca. 1500-year-old mural painting, and emended description of the genus Naumannella
Description of Naumannella cuiyingiana sp nov., isolated from a ca. 1500-year-old mural painting, and emended description of the genus Naumannella
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Naumannella cuiyingiana sp nov. 的描述，从 ca. 中分离出来。
DOI：
10.1099/ijsem.0.001964
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发表时间：
2017
影响因子：
2.8
通讯作者：
Feng Huyuan
中科院分区：
文献类型：
--
作者：
Tian Tian;Wu Fasi;Ma Yantian;Xiang Ting;Ma Wenxia;Hu Weigang;Wu Guangwen;An Lizhe;Wang Wanfu;Feng Huyuan
菌株AFT 2 T是从一幅壁画中分离得到的。1500年前的古墓位于中国山西省。分离株为革兰氏染色阳性、非运动、非孢子形成、需氧、椭圆形至短杆状细菌，形成白色菌落。基于16 S rRNA基因序列的系统发育分析表明，菌株AFT 2 T与Naumannella halotoleransWS 4616 T（=DSM 24323 T）的亲缘关系最近，为一个单系分支，亲缘关系达97.01%。 基因组DNA的G+C含量为71.97mol%，与N. 耐盐剂DSM 24323 T。细胞脂肪酸主要为antiso-C15：0（55.32%），呼吸醌只有MK-9（H4）。 极性脂由磷脂酰甘油、二磷脂酰甘油、2种未知磷脂和5种未知糖脂组成，在细胞壁肽聚糖（A3γ型）中检测到11-二氨基庚二酸，全细胞糖由核糖、甘露糖、阿拉伯糖和半乳糖组成。根据AFT 2 T菌株的表型特征和系统发育特征，将其分类为Naumannella属的一个新种，命名为Naumannella cuiyingianasp。11月。模式菌株为AFT 2 T（=CCTCC AB 2015428 T =DSM 103164 T）。
Strain AFT2Twas isolated from a mural painting sample from aca. 1500-year-old tomb located in Shanxi Province, China. The isolate was a Gram-stain-positive, non-motile, non-spore-forming, aerobic and oval to short-rod-shaped bacterium that formed white-pigmented colonies. Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain AFT2Twas most closely (97.01 %) correlated and formed a monophyletic clade withNaumannella halotoleransWS4616T(=DSM 24323T). The G+C content of the genomic DNA was 71.97 mol%, and the strain showed 37.27 % DNA–DNA relatedness toN. halotoleransDSM 24323T. The major cellular fatty acid was anteiso-C15 : 0(55.32 %), and MK-9(H4) was the only respiratory quinone. The polar lipids comprised phosphatidylglycerol, diphosphatidylglycerol, two unknown phospholipids and five unknown glycolipids.ll-Diaminopimelic acid was detected in the cell-wall peptidoglycan (type A3γ), and the whole-cell sugars consisted of ribose, mannose, arabinose and galactose. On the basis of its phenotypic and phylogenetic characteristics, it is proposed that strain AFT2Tshould be classified as a representative of a novel species of the genusNaumannella, for which the nameNaumannella cuiyingianasp. nov. is proposed. The type strain is AFT2T(=CCTCC AB 2015428T=DSM 103164T).