Comprehensive metabolite profiling of phyA phyB phyC triple mutants to reveal their associated metabolic phenotype in rice leaves
Comprehensive metabolite profiling of phyA phyB phyC triple mutants to reveal their associated metabolic phenotype in rice leaves
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DOI：
10.1016/j.jbiosc.2009.03.010
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发表时间：
2009-08-01
影响因子：
2.8
通讯作者：
Kobayashi, Akio
中科院分区：
文献类型：
--
作者：
Jumtee, Kanokwan;Okazawa, Atsushi;Kobayashi, Akio
光敏色素光感受器在植物的整个生命周期中调控着植物的生长发育。水稻（Oryza sativa）具有三种光敏色素：phyA、phyB和phyC。对每种光敏色素零突变体的生理、遗传和生化分析揭示了每种光敏色素在水稻中的功能。然而，很少有研究探讨光敏色素信号传导与代谢的关系。本研究采用气相色谱-飞行时间质谱（GC/TOF-MS）非靶向代谢物分析和毛细管电泳-电喷雾质谱（CE/ESI-MS）靶向代谢物分析对水稻phyA phyB phyC三突变体的代谢变化进行了研究。主成分分析（PCA）可以清楚地观察到phyA phyB phyC三突变体与野生型（WT）、幼叶与成熟叶之间不同的代谢特征。代谢物谱显示，与WE相比，phyA phyB phyC三突变体叶片中氨基酸、有机酸、糖、磷酸糖和核苷酸的积累量较高，尤其是在幼叶中。在phyA phyB phyC三突变体的幼叶中，观察到葡萄糖（53.4倍）、果糖（42.5倍）和半乳糖（24.5倍）等单糖的过度积累。这些代谢表型表明，在phyA phyB phyC三突变体中，糖代谢、碳分配、糖转运或这些因素的某些组合受到损害，相反，光敏色素在糖代谢中起关键作用。(C) 2009，生物技术学会，日本。版权所有。
The phytochrome photoreceptors regulate plant growth and development throughout their life cycle. Rice (Oryza sativa) possesses three phytochromes, phyA, phyB, and phyC. Physiological, genetic, and biochemical analyses of null mutants of each phytochrome have revealed the function of each in rice. However, few studies have investigated the relationship between phytochrome signaling and metabolism. In the present study, non-targeted metabolite analysis by gas chromatography time-of-flight mass spectrometry (GC/TOF-MS) and targeted metabolite analysis by capillary electrophoresis electrospray ionization mass spectrometry (CE/ESI-MS) were employed to investigate metabolic changes in rice phyA phyB phyC triple mutants. Distinct metabolic profiles between phyA phyB phyC triple mutants and the wild type (WT), as well as those between young and mature leaf blades, could be clearly observed by principal component analysis (PCA). The metabolite profiles indicated high accumulation of amino acids, organic acids, sugars, sugar phosphates, and nucleotides in the leaf blades of phyA phyB phyC triple mutants, especially in the young leaves, compared with those in the WE Remarkable overaccumulation of monosaccharide, such as glucose (53.4-fold), fructose (42.5-fold), and galactose (24.5-fold), was observed in young leaves of phyA phyB phyC triple mutants. These metabolic phenotypes suggest that sugar metabolism, carbon partitioning, sugar transport, or some combination of these is impaired in the phyA phyB phyC triple mutants, and conversely, that phytochromes have crucial roles in sugar metabolism. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.