Multiple requirements for Hes1 during early eye formation
Multiple requirements for Hes1 during early eye formation
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DOI：
10.1016/j.ydbio.2005.06.010
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发表时间：
2005-08-15
影响因子：
2.7
通讯作者：
Brown, NL
中科院分区：
文献类型：
--
作者：
Lee, HY;Wroblewski, E;Brown, NL
在胚胎发生过程中，多个发育过程通过它们精确的时间调控被整合在一起。Hes1是一种调节哺乳动物视网膜神经发生时间的转录抑制因子。然而，Hes1在早期眼睛发育中的作用尚未得到很好的定义。在这里，我们发现Hes1在晶状体、视泡、杯和色素上皮中表达，并且是这些组织正常生长、形态发生和分化所必需的。由于整个眼睛都需要Hes1，我们研究了它与Pax6的相互作用。Hes1-Pax6双突变胚胎是无眼的，这表明这些基因是视神经囊泡的初始形态发生和生长所必需的。在Hes1突变体中，Math5的表达随着视网膜神经节细胞、无突细胞和水平神经元的形成而早熟。与Pax6和Hes1在形态发生过程中的明显协同作用相反，每个基因独立调控Math5和RGC的发生。综上所述，这些研究表明Hes1和Pax6一样，在视觉发育过程中同时调控多个发育过程。(c) 0 2005 Elsevier Inc.版权所有。
During embryogenesis, multiple developmental processes are integrated through their precise temporal regulation. Hes1 is a transcriptional repressor that regulates the timing of mammalian retinal neurogenesis. However, roles for Hes1 in early eye development have not been well defined. Here, we show that Hes1 is expressed in the forming lens, optic vesicle, cup, and pigmented epithelium and is necessary for proper growth, morphogenesis, and differentiation of these tissues. Because Hes1 is required throughout the eye, we investigated its interaction with Pax6. Hes1-Pax6 double mutant embryos are eyeless suggesting these genes are coordinately required for initial morphogenesis and outgrowth of the optic vesicle. In Hes1 mutants, Math5 expression is precocious along with retinal ganglion cell, amacrine, and horizontal neuron formation. In contrast to apparent cooperativity between Pax6 and Hes1 during morphogenesis, each gene regulates Math5 and RGC genesis independently. Together, these studies demonstrate that Hes1, like Pax6, simultaneously regulates multiple developmental processes during optic development.(c) 0 2005 Elsevier Inc. All rights reserved.