Benzaldehyde as an insecticidal, antimicrobial, and antioxidant compound produced by Photorhabdus temperata M1021
Benzaldehyde as an insecticidal, antimicrobial, and antioxidant compound produced by Photorhabdus temperata M1021
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DOI：
10.1007/s12275-015-4632-4
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发表时间：
2015-02-01
影响因子：
3
通讯作者：
Shin, Jae-Ho
中科院分区：
文献类型：
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作者：
Ullah, Ihsan;Khan, Abdul Latif;Shin, Jae-Ho
Photorhabdus temperata M1021分泌有毒化合物，通过阻止免疫反应杀死昆虫宿主。本研究的目的是通过生物测定指导的分级分离，从温度P.temperataM1021的培养提取物中纯化杀虫和抗菌化合物。温性假单胞菌M1021的乙酸乙酯（EtOAc）提取物在72 h内对大蜡螟幼虫表现出100%的死亡率。此外，乙酸乙酯提取物和经柱层析纯化的活性化合物1对酚氧化酶的抑制率分别达到60%和80%。H-1和C-13 NMR谱的分析揭示了纯化合物为“苯甲醛”的身份。该化合物具有杀虫活性。在注射8 mM苯甲醛后108小时，观察到100%的昆虫死亡率。在PO抑制测定中，发现4、6和8 mM浓度的苯甲醛分别抑制PO活性约15%、42%和80%。此外，与对照相比，结节形成被4、6和8 mM苯甲醛显著抑制（P < 0.05）。此外，苯甲醛被发现有很大的抗氧化活性，最大的抗氧化活性为52.9%，与对照相比，在8 mM苯甲醛。通过MIC值评估抗微生物活性，对于细菌菌株，MIC值范围为6 mM-10 mM，对于真菌菌株，MIC值范围为8 mM-10 mM。结果表明，苯甲醛可用于开发新型农用杀虫剂。
The Photorhabdus temperata M1021 secretes toxic compounds that kill their insect hosts by arresting immune responses. Present study was aimed to purify the insecticidal and antimicrobial compound(s) from the culture extract of P. temperata M1021 through bioassay guided fractionation. An ethyl acetate (EtOAc) extract of the P. temperata M1021 exhibited 100% mortality in Galleria mellonella larvae within 72 h. In addition, EtOAc extract and bioactive compound 1 purified form the extract through to column chromatography, showed phenol oxidase inhibition up to 60% and 80% respectively. The analysis of H-1 and C-13 NMR spectra revealed the identity of pure compound as "benzaldehyde". The benzaldehyde showed insecticidal activity against G. mellonella in a dose-dependent manner and 100% insect mortality was observed at 108 h after injection of 8 mM benzaldehyde. In a PO inhibition assay, 4, 6, and 8 mM concentrations of benzaldehyde were found to inhibit PO activity about 15%, 42%, and 80% respectively. In addition, nodule formation was significantly (P < 0.05) inhibited by 4, 6, and 8 mM of benzaldehyde as compare to control. Moreover, benzaldehyde was found to have great antioxidant activity and maximum antioxidant activity was 52.9% at 8 mM benzaldehyde as compare to control. Antimicrobial activity was assessed by MIC values ranged from 6 mM 10 mM for bacterial strains and 8 mM to 10 mM for fungal strains. The results suggest that benzaldehyde could be applicable for developing novel insecticide for agriculture use.