Endogenous nitric oxide synthase inhibitors in endothelial cells, endothelin-1 within the vessel wall, and intimal hyperplasia in perimenopausal human uterine arteries
Endogenous nitric oxide synthase inhibitors in endothelial cells, endothelin-1 within the vessel wall, and intimal hyperplasia in perimenopausal human uterine arteries
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DOI：
10.1097/00005344-200202000-00005
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发表时间：
2002-02-01
影响因子：
3
通讯作者：
Azuma, H
中科院分区：
文献类型：
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作者：
Beppu, M;Obayashi, S;Azuma, H
本实验旨在研究围绝经期人子宫动脉产生一氧化氮的能力、内皮细胞中N-G-单甲基-L-精氨酸（L-NMMA）和不对称N-G，N-G-二甲基-L-精氨酸（ADMA）的浓度、血管壁内内皮素-1和内膜增生程度（内膜/中膜比）。根据基于基础环GMP水平的初步分类，16条动脉可分为I组和II组，每组8条。组I的核苷酸净产量显著高于组II。Ⅱ组L-NMMA + ADMA浓度及ET-1含量均显著高于Ⅱ组。第I组的所有标本在组织学上均正常，而第II组标本中观察到轻度至重度内膜增生。尽管在内膜/中膜比率、L-NMMA + ADMA和内皮素-1（各n=35）中可检测到相当大的个体差异，但三个参数之间存在显著的正相关性，表明内膜增生随着L-NMMA + ADMA和内皮素-1的增加而增加。这些结果表明，内皮细胞内源性一氧化氮合酶抑制剂和血管壁内皮素-1是内膜增生的重要标志物。
The present experiments were designed to investigate the ability to produce nitric oxide, concentrations of N-G-monomethyl-L-arginine (L-NMMA), and asymmetric N-G,N-G-dimethyl-L-arginine (ADMA) in endothelial cells, endothelin-1 within the vessel wall, and the degree of intimal hyperplasia (intima/media ratio) in perimenopausal human uterine arteries. According to the tentative classification based on basal cyclic GMP levels, 16 arteries could be grouped into groups I and II consisting of eight each. Net production of the nucleotide was significantly higher in group I than that in group II. Concentration of L-NMMA plus ADMA and endothelin-1 content were significantly higher in group II. All specimens from group I were histologically normal, whereas mild to severe intimal hyperplasia was observed in group II specimens. Although considerable individual variations were detectable in the intima/media ratio, L-NMMA plus ADMA and endothelin-1 (n=35 each), there were significant and positive correlations between three parameters, indicating that intimal hyperplasia became greater as L-NMMA plus ADMA and endothelin-1 were increased. These results suggest that endogenous nitric oxide synthase inhibitors in endothelial cells and endothelin-1 within the vessel wall are important markers of intimal hyperplasia.