Association of symptoms with gastrointestinal microbiota in irritable bowel syndrome
Association of symptoms with gastrointestinal microbiota in irritable bowel syndrome
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DOI：
10.3748/wjg.v16.i36.4532
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发表时间：
2010-09-28
影响因子：
4.3
通讯作者：
Palva, Airi
中科院分区：
文献类型：
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作者：
Malinen, Erja;Krogius-Kurikka, Lotta;Palva, Airi
目的：调查肠易激综合征 (IBS) 自我报告症状与胃肠道 (GI) 微生物群组成之间的相关性。方法：从总共 44 名诊断为 IBS 的受试者中收集粪便样本。他们的症状通过针对 IBS 患者进行调整的经过验证的炎症性肠病调查问卷进行监测。应用十三种定量实时聚合酶链反应测定来评估胃肠道微生物群组成。真细菌和胃肠道细菌属（双歧杆菌、乳杆菌和韦荣氏菌）、类群（球状梭菌/直肠真杆菌、Desulfovibrio desulfuricans）和不同的细菌系统型[与链状双歧杆菌、串状梭菌最接近的 16S rDNA 序列相似性， 对疑似与 IBS 相关的 aerofaciens (C. aerofaciens)、Coprococus eutactus (C. eutactus)、扭瘤胃球菌和弓链球菌]进行了量化。研究了选定细菌群或系统型的数量或存在/不存在数据与各种 IBS 相关症状之间的相关性。结果：观察到受试者自我报告的症状与某些胃肠道细菌的存在或数量之间的关联。扭瘤胃球菌 (R.torques) 样（16S rRNA 基因序列相似性为 94%）系统发育型与肠道症状的严重程度相关。此外，在检测到 R. Torks 94% 的 IBS 受试者中，C cocleatum 88%、C aerofaciens-like 和 C eutactus 97% 系统发育型的数量显着减少。关于受试者体重对胃肠道微生物群的影响，还进行了有趣的观察，这些微生物群涉及 C aerofaciens 样系统型、双歧杆菌属。结论：看似影响症状评分的细菌不太可能是 IBS 的根本原因或治愈方法，但它们可能作为该疾病的生物标志物。 (C)2010年百事登。版权所有。
AIM: To investigate the correlations between self-reported symptoms of irritable bowel syndrome (IBS) and the gastrointestinal (GI) microbiota composition.METHODS: Fecal samples were collected from a total of 44 subjects diagnosed with IBS. Their symptoms were monitored with a validated inflammatory bowel disease questionnaire adjusted for IBS patients. Thirteen quantitative real-time polymerase chain reaction assays were applied to evaluate the GI microbiota composition. Eubacteria and GI bacterial genera (Bifidobacterium, Lactobacillus and Veillonella), groups (Clostridium coccoides/Eubacterium rectale, Desulfovibrio desulfuricans) and distinct bacterial phylotypes [closest 16S rDNA sequence resemblance to species Bifidobacterium catenulatum, Clostridium cocleatum, aerofaciens (C. aerofaciens), Coprococcus eutactus (C. eutactus), Ruminococcus torques and Streptococcus bow's] with a suspected association with IBS were quantified. Correlations between quantities or presence/absence data of selected bacterial groups or phylotypes and various IBS-related symptoms were investigated.RESULTS: Associations were observed between subjects' self-reported symptoms and the presence or quantities of certain GI bacteria. A Ruminococcus torques (R. torques)-like (94% similarity in 16S rRNA gene sequence) phylotype was associated with severity of bowel symptoms. Furthermore, among IBS subjects with R. torques 94% detected, the amounts of C cocleatum 88%, C aerofaciens-like and C eutactus 97% phylotypes were significantly reduced. Interesting observations were also made concerning the effect of a subject's weight on GI microbiota with regard to C aerofaciens-like phylotype, Bifidobacterium spp. and Lactobacillus spp.CONCLUSION: Bacteria seemingly affecting the symptom scores are unlikely to be the underlying cause or cure of IBS, but they may serve as biomarkers of the condition. (C) 2010 Baishideng. All rights reserved.