Comparison of telemetric and tail-cuff blood pressure monitoring in adrenocorticotrophic hormone-treated rats
Comparison of telemetric and tail-cuff blood pressure monitoring in adrenocorticotrophic hormone-treated rats
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DOI：
10.1046/j.1440-1681.2001.03531.x
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发表时间：
2001-10-01
影响因子：
2.9
通讯作者：
Whitworth, JA
中科院分区：
文献类型：
--
作者：
Fraser, TB;Turner, SW;Whitworth, JA
1. 本研究的目的是验证遥测血压 (BP) 监测系统对促肾上腺皮质激素 (ACTH) 治疗和假治疗大鼠尾部袖带血压的影响。在统计分析中，我们首先测试了与生理盐水相比，ACTH 治疗 10 天对收缩压 (SBP) 是否有可检测到的影响。其次，我们比较了遥测和尾套测量的结果，第三，我们开发了一种新方法来估计两种技术的相对功率。2．将 23 只雄性 Sprague-Dawley 大鼠随机分为两组：(i) ACTH（每天 100 杯/公斤，皮下注射；n = 12）治疗；或 (ii) 假处理（0.9% 盐水，s.c.；n = 11）。通过遥测系统（每 2 分钟采样 10 秒）连续测量收缩压 4 小时（n = 16）或 30 分钟（n = 23），并且每天通过间接尾套法测量收缩压（n = 23）。组内和组间数据进行比较；然后进行普通最小乘积（OLP）回归分析以测试两种方法之间的偏差。还进行了样本量/功效估计。3．与使用尾套法的 14 mmHg (P < 0.001) 相比，促肾上腺皮质激素治疗使遥测 SBP 提高了 11 mmHg (P < 0.001)。回归分析显示，两种测量方法之间不存在固定或比例偏差。功效计算表明，最小样本量为 6 时，尾套遥测的功效为 0.84/0.86 = 0.98。 4 小时与 30 分钟血压测量的功效为 0.99/0.82 = 1.2.4。遥测得出的结果与尾套法非常相似。遥测技术允许更长的测量时间，为研究提供更大的动力，从而需要更少的动物。
1. The aim of the present study was to validate a telemetric blood pressure (BP) monitoring system against tail-cuff blood pressure in both adrenocorticotrophic hormone (ACTH)- and sham-treated rats. In the statistical analyses, we first tested whether there was a detectable effect on systolic blood pressure (SBP) of 10 days treatment with ACTH compared with saline. Second, we compared results of telemetered and tail-cuff measurements and, third, we developed a novel method for estimating the relative power of the two techniques.2. Twenty-three male Sprague-Dawley rats were randomly divided into two groups: (i) ACTH (100 mug/kg per day, s.c.; n = 12) treated; or (ii) sham treated (0.9% saline, s.c.; n = 11). Systolic BP was measured by the telemetric system (sampled for 10 s every 2 min) continuously for 4 h (n = 16) or for 30 min (n = 23) and also by the indirect tail-cuff method daily (n = 23). Data were compared within and between groups; ordinary least products (OLP) regression analysis was then performed to test for bias between the two methods. Sample size/power estimations were also performed.3. Adrenocorticotrophic hormone treatment raised telemetered SBP by 11 mmHg (P < 0.001) compared with 14 mmHg (P < 0.001) using the tail-cuff method. There was no fixed or proportional bias between the two methods of measurement, as shown by regression analysis. Power calculations indicate that a minimum sample size of six gives a power of telemetered to tail-cuff of 0.84/0.86 = 0.98. The power of 4 h versus 30 min BP measurements was 0.99/0.82 = 1.2.4. Telemetry gave very similar results to the tail-cuff method. Telemetry allows for a longer period of measurement, giving greater power to the study so that fewer animals are needed.