Gentacoll® hampers epithelialisation and neovascularisation in excisional wounds in hairless mice
Gentacoll® hampers epithelialisation and neovascularisation in excisional wounds in hairless mice
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DOI：
10.1080/02844319850158732
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发表时间：
1998-06-01
影响因子：
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通讯作者：
Hjortdal, VE
中科院分区：
文献类型：
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作者：
Bang, K;Sampram, E;Hjortdal, VE
我们的目的是分析Gentacoll（R）对伤口愈合中上皮形成和新生血管形成率的影响。在24只无毛纯合小鼠（n = 48）的每只耳的背侧上产生直径为2.25 mm的标准化圆形全厚度真皮伤口。软骨层保持完整。以随机设盲方式使用含庆大霉素的牛胶原蛋白植入物（Gentacoll（R））（n = 17）、不含庆大霉素的牛胶原蛋白植入物（n = 15）和硅胶膜（n = 16）治疗伤口。在伤口形成后立即通过活体视频显微镜和计算机化面积测量法直接测量上皮化和新血管形成，并且每隔一天测量一次，直到伤口闭合。用Gentacoll（R）（n=17）处理的伤口中只有5个完全上皮化;平均上皮形成时间（SEM）为22.8（1.6）天，显著长于不含庆大霉素的对照组（n = 15），其相应的数字为14.5（0.6）天。在用Gentacoll（R）治疗的9个伤口中，伤口床中的耳软骨穿孔，两个伤口出现严重炎症，随后自残。庆大霉素组所有伤口的新生血管形成均不完全，而对照组在25.3（0.7）天内完成，对照组使用不含庆大霉素的植入物治疗。在硅胶治疗组（n = 16）中，12.7（0.7）天完成上皮形成，25.1（0.5）天完成新血管形成。单独使用胶原蛋白或硅胶治疗的伤口均未显示出与Gentacoll（R）治疗耳相似的反应。Gentacoll（R）阻碍上皮形成和新生血管形成，并可能损伤暴露的软骨。
Our aim was to analyse the effect of Gentacoll(R) on the rate of epithelialisation and neovascularisation in wound healing. Standardised circular full thickness dermal wounds 2.25 mm in diameter were created on the dorsum of each ear on 24 hairless homozygous mice (n = 48). The cartilaginous layer was left intact. The wounds were treated in a randomised blinded fashion with bovine collagen implants with gentamicin (Gentacoll(R)) (n = 17); bovine collagen implants without gentamicin (n = 15); and Silicone film (n = 16). Epithelialisation and neovascularisation were measured directly by intravital video-microscopy and computerised planimetry immediately after the wounds had been made and every third day until the wounds closed, Only five of the wounds treated with Gentacoll(R) (n=17) epithelialised completely; and their mean (SEM) epithelialisation time was 22.8 (1.6) days, significantly longer than controls without gentamicin (n = 15) for which the corresponding figures were 14.5 (0.6) days. In nine wounds treated with Gentacoll(R) the ear cartilage in the wound bed perforated and two wounds developed severe inflammation which was followed by self-mutilation. Neovascularisation was incomplete in all of the wounds in the Gentacoll(R) group, whereas it was completed by 25.3 (0.7) days in the control group treated with implants without gentamicin. In the silicone treated group (n = 16), epithelialisation was completed by 12.7 (0.7) days and neovascularisation by 25.1 (0.5) days. None of wounds treated with collagen or silicone alone showed reactions similar to the Gentacoll(R)-treated ears. Gentacoll(R) hampers epithelialisation and neovascularisation, and might damage exposed cartilage.