Two cultivated legume plants reveal the enrichment process of the microbiome in the rhizocompartments
Two cultivated legume plants reveal the enrichment process of the microbiome in the rhizocompartments
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两种栽培的豆科植物揭示了根际微生物组的富集过程
DOI：
10.1111/mec.14027
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发表时间：
2017
影响因子：
4.9
通讯作者：
Wei Gehong
中科院分区：
文献类型：
--
作者：
Xiao Xiao;Chen Weimin;Zong Le;Yang Jun;Jiao Shuo;Lin Yanbing;Wang Entao;Wei Gehong
利用高通量测序技术分析了大豆和苜蓿根区（根瘤内生菌、根内生菌、根际和根区）的微生物组，以研究豆科植物、微生物和土壤类型之间的相互作用。在四个根区-结核内圈、根内圈、根圈和根区-中观察到植物对微生物群有明显的分级过滤，不同根区中微生物群落组成的显著差异证明了这一点。根际和根区微生物群落受土壤类型的影响较大，根瘤和根内生菌主要由植物种类决定。根瘤菌群多样，苜蓿根瘤菌群以Ensifer-Bradyrhizobium为主，大豆根瘤菌群以Ensifer-Bradyrhizobium为主。非共生根瘤内生菌主要为变形菌门、放线菌门、厚壁菌门和拟杆菌门。根系微生物群落的变化也受到植物生长阶段的影响。总之，这项研究表明，结核内生菌的富集过程遵循分级过滤，结核内生菌中的细菌群落根据植物物种而变化。
The microbiomes of rhizocompartments (nodule endophytes, root endophytes, rhizosphere and root zone) in soya bean and alfalfa were analysed using high‐throughput sequencing to investigate the interactions among legume species, microorganisms and soil types. A clear hierarchical filtration of microbiota by plants was observed in the four rhizocompartments – the nodule endosphere, root endosphere, rhizosphere and root zone – as demonstrated by significant variations in the composition of the microbial community in the different compartments. The rhizosphere and root zone microbial communities were largely influenced by soil type, and the nodule and root endophytes were primarily determined by plant species. Diverse microbes inhabited the root nodule endosphere, and the corresponding dominant symbiotic rhizobia belonged toEnsiferfor alfalfa andEnsifer–Bradyrhizobiumfor soya bean. The nonsymbiotic nodule endophytes were mainly Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes. The variation in root microbial communities was also affected by the plant growth stage. In summary, this study demonstrated that the enrichment process of nodule endophytes follows a hierarchical filtration and that the bacterial communities in nodule endophytes vary according to the plant species.