Microflow1, A Sheathless Fiber-Optic Flow Cytometry Biomedical Platform: Demonstration Onboard the International Space Station
Microflow1, A Sheathless Fiber-Optic Flow Cytometry Biomedical Platform: Demonstration Onboard the International Space Station
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DOI：
10.1002/cyto.a.22427
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发表时间：
2014-04-01
期刊：
影响因子：
3.7
通讯作者：
Cohen, Luchino Y.
中科院分区：
文献类型：
--
作者：
Dubeau-Laramee, Genevieve;Riviere, Christophe;Cohen, Luchino Y.
设计了一种基于光纤的流式细胞仪平台，用于构建便携式和坚固耐用的空间应用仪器。Microflow 1的核心是一个独特的光纤流量池，安装在流体系统中，光纤连接到源和检测通道。Microflow 1工程单元首先在标准实验室环境中以商用流式细胞仪为基准进行测试和基准测试。在国际空间站上运行新平台之前，进行了抛物线飞行运动测试，以确定Microflow 1的S在失重状态下的表现。Microflow 1具有与商业系统相当的性能，在失重(微重力)下运行显著且稳健。Microflow 1支持在空间环境中独立于重力水平的免疫表型分析以及基于微珠的多路细胞因子分析。这里提供的结果提供了证据，证明这种光纤细胞仪技术与空间环境本质上是兼容的，对分析性能的影响可以忽略不计。由Wiley出版-期刊出版公司(C)2013年加拿大政府
A fiber-optic based flow cytometry platform was designed to build a portable and robust instrument for space applications. At the core of the Microflow1 is a unique fiber-optic flow cell fitted to a fluidic system and fiber coupled to the source and detection channels. A Microflow1 engineering unit was first tested and benchmarked against a commercial flow cytometer as a reference in a standard laboratory environment. Testing in parabolic flight campaigns was performed to establish Microflow1's performance in weightlessness, before operating the new platform on the International Space Station. Microflow1 had comparable performances to commercial systems, and operated remarkably and robustly in weightlessness (microgravity). Microflow1 supported immunophenotyping as well as microbead-based multiplexed cytokine assays in the space environment and independently of gravity levels. Results presented here provide evidence that this fiber-optic cytometer technology is inherently compatible with the space environment with negligible compromise to analytical performance. Published by Wiley-Periodicals, Inc. (c) 2013 Government of Canada