GROWTH FACTOR-INDUCED ACCELERATION OF TISSUE-REPAIR THROUGH DIRECT AND INDUCTIVE ACTIVITIES IN A RABBIT DERMAL ULCER MODEL
GROWTH FACTOR-INDUCED ACCELERATION OF TISSUE-REPAIR THROUGH DIRECT AND INDUCTIVE ACTIVITIES IN A RABBIT DERMAL ULCER MODEL
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DOI：
10.1172/jci115048
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发表时间：
1991-02-01
影响因子：
15.9
通讯作者：
DEUEL, TF
中科院分区：
文献类型：
--
作者：
MUSTOE, TA;PIERCE, GF;DEUEL, TF
多肽生长因子在促进创面愈合和指导创面不同组织反应的特异性和序列方面的作用尚不清楚。我们研究了四种生长因子对放置在兔耳无血管基底部的新型全层皮肤溃疡愈合率的影响。创伤模型排除了明显的伤口收缩，需要新的肉芽组织和上皮细胞才能愈合向心起源。创伤时局部应用5-mU-g(7-31pmol/mm2)的血小板衍生生长因子-B链(PDGF-BB)、碱性成纤维细胞生长因子(BFGF)和表皮生长因子(EGF)可使治疗创面完全再上皮化增加一倍(PDGF-BB，P=0.02卡方分析；bFGF，P=0.04；EGF，P=0.05)，转化生长因子(TGF)-β1显著抑制再上皮化(P=0.05)。血小板衍生生长因子-BB和转化生长因子-β-1均能增加创面新生肉芽组织的深度和面积(P<；0.005)，增加成纤维细胞的流入，增加创面新基质的沉积。PDGF-BB处理7d的创面外植体的代谢活性远高于对照组，DNA中的[H-3]胸腺嘧啶核苷含量增加了473%(P=0.05)，胶原酶敏感蛋白中的[H-3]亮氨酸和[H-3]Pro含量显著增加(P=0.04)。结果表明，多肽生长因子对兔全层溃疡的愈合有显著的、选择性的积极影响。
The roles of polypeptide growth factors in promoting wound healing and in directing the specificity and sequence of responses of different tissues in wounds are little understood. We investigated the influence of four growth factors on the rates of healing of a novel full thickness dermal ulcer placed on an avascular base in the rabbit ear. The wound model precludes significant wound contraction and requires new granulation tissue and epithelial cells for healing to originate centripetally. 5-mu-g (7-31 pmol/mm2) of platelet-derived growth factor-B chain (PDGF-BB), basic fibroblast growth factor (bFGF), and epidermal growth factor (EGF) applied locally at the time of wounding resulted in a twofold increase in complete reepithelialization of treated wounds (PDGF-BB, P = 0.02 chi square analysis; bFGF, P = 0.04; EGF, P = 0.05); transforming growth factor (TGF)-beta-1 significantly inhibited reepithelialization (P = 0.05). Both PDGF-BB and TGF-beta-1 uniquely increased the depth and area of new granulation tissue (P < 0.005), the influx of fibroblasts, and the deposition of new matrix into wounds. Explants from 7-d old PDGF-BB-treated wounds remained metabolically far more active than controls, incorporating 473% more [H-3]thymidine into DNA (P = 0.05) and significantly more [H-3]leucine and [H-3]proline into collagenase-sensitive protein (P = 0.04). The results establish that polypeptide growth factors have significant and selective positive influences on healing of full thickness ulcers in the rabbit.