Quantum dot encoding of aptamer-linked nanostructures for one-pot simultaneous detection of multiple analytes
Quantum dot encoding of aptamer-linked nanostructures for one-pot simultaneous detection of multiple analytes
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DOI：
10.1021/ac070055k
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发表时间：
2007-06-01
影响因子：
7.4
通讯作者：
Lu, Yi
中科院分区：
文献类型：
--
作者：
Liu, Juewen;Lee, Jung Heon;Lu, Yi
分析化学中的一个主要挑战是多种分析物的多重感测，其中每种分析物显示不同的信号。为了应对这样的挑战，在525和585 nm处发射的量子点被用于编码分别对腺苷和可卡因敏感的适体连接的纳米结构。除了量子点之外，纳米结构还包含用作猝灭剂的金纳米颗粒。目标分析物的添加使纳米结构解体并导致量子点的发射增加。同时比色和荧光检测和定量的两种分子在一锅证明。
One major challenge in analytical chemistry is multiplex sensing of a number of analytes with each analyte displaying a different signal. To meet such a challenge, quantum dots that emit at 525 and 585 nm are used to encode aptamer-linked nanostructures sensitive to adenosine and cocaine, respectively. In addition to quantum dots, the nanostructures also contain gold nanoparticles that serve as quenchers. Addition of target analytes disassembles the nanostructures and results in increased emission from quantum dots. Simultaneous colorimetric and fluorescent detection and quantification of both molecules in one pot is demonstrated.