31P-magnetic resonance spectroscopy of leg muscle metabolism during exercise in children and adults.
31P-magnetic resonance spectroscopy of leg muscle metabolism during exercise in children and adults.
复制标题
儿童和成人运动期间腿部肌肉代谢的 31P 磁共振波谱。
DOI：
10.1152/jappl.1993.74.5.2214
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Cooper,DM
中科院分区：
文献类型：
--
作者：
Zanconato,S;Buchthal,S;Barstow,TJ;Cooper,DM
儿童和成人对高强度运动的气体交换反应不同。我们假设这些发现与儿童无氧 ATP 供应较低有关。因此，我们预测运动期间肌肉高能磷酸盐代谢会成熟。为了验证这一假设，我们使用 31P 核磁共振波谱测量了 10 名儿童和 8 名成人在休息和渐进运动期间小腿肌肉 Pi、磷酸肌酸 (PCr) 和 pH 值。儿童和成人之间在静息状态下的 pH 值和 Pi/PCr 值没有差异。与儿童相比，运动导致成人的 Pi/PCr 增加更多（P < 0.001），pH 降低（P < 0.0001）。六名成人和五名儿童在运动过程中表现出 Pi/PCr 增加和 pH 下降从缓慢到较快的转变。在 Pi/PCr 和 pH 作为工作率函数的初始慢相斜率方面，两组之间没有发现显着差异。相反，在快速阶段，成人的 Pi/PCr 增加（斜率：成人 23.6 +/- 9.8，儿童 10.7 +/- 2.5；P < 0.05）和 pH 值下降（斜率：成人 -6.0 +/- 1.9，儿童 -3.7 +/- 1.2；P < 0.05）比儿童更快。总之，高强度运动会导致儿童和成人之间的 Pi/PCr 和 pH 动力学不同。这些结果表明，与成人相比，儿童在高强度运动期间通过无氧代谢途径影响 ATP 再磷酸化的能力较差。
Gas exchange response to high-intensity exercise differs in children and adults. We hypothesized that these findings are related to a lower anaerobic ATP supply in children. We predicted therefore a maturation of muscle high-energy phosphate metabolism during exercise. To test this hypothesis, we measured calf muscle Pi, phosphocreatine (PCr), and pH with 31P-nuclear magnetic resonance spectroscopy during rest and progressive exercise in 10 children and 8 adults. No differences were found at rest in pH and Pi/PCr between children and adults. Exercise resulted in a greater increase in Pi/PCr (P < 0.001) and decrease in pH (P < 0.0001) in adults than in children. Six adults and five children exhibited a transition from a slow to a faster rate of Pi/PCr increase and pH decrease during exercise. No significant differences were found between the two groups in the initial slow-phase slopes of Pi/PCr and pH as a function of work rate. In contrast, during the fast phase, Pi/PCr increased (slope: adults 23.6 +/- 9.8, children 10.7 +/- 2.5; P < 0.05) and pH decreased (slope: adults -6.0 +/- 1.9, children -3.7 +/- 1.2; P < 0.05) more rapidly in adults than in children. In conclusion, high-intensity exercise results in different kinetics of Pi/PCr and pH between children and adults. These results suggest that children are less able than adults to affect ATP rephosphorylation by anaerobic metabolic pathways during high-intensity exercise.