Evaluation of 1.5 microM reversed phase nonporous silica in packed capillary electrochromatography and application in pharmaceutical analysis.
Evaluation of 1.5 microM reversed phase nonporous silica in packed capillary electrochromatography and application in pharmaceutical analysis.
复制标题
1.5 µM 反相无孔二氧化硅在填充毛细管电色谱中的评价及其在药物分析中的应用。
DOI：
10.1002/(sici)1522-2683(19990801)20:12
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Stobaugh,JF
中科院分区：
文献类型：
--
作者：
Angus,PD;Demarest,CW;Catalano,T;Stobaugh,JF
Reversed‒phase nonporous silica (RP‒NPS) of 1.5 μmdpis employed to demonstrate rapid and efficient separations in packed capillary electrochromatography (CEC). Two methods for packing capillaries and two techniques to manufacture frits used to hold the packing in place are evaluated for their effect upon separation performance using polyaromatic hydrocarbons (PAHs) and polar neutral pharmaceutical compounds. Attention is given to conditioning of the packed capillaries for high efficiency separations without necessity for sodium dodecyl sulfate (SDS). Separation conditions for the nonporous materials were modified from those previously determined on pourous reversed‒phase silica. Feasibility for method development and validation of a parent pharmaceutical compound and related impurities in the range of 0.1—120% of a 5 mg/mL concentration was assessed and reported. An approach to improving detection sensitivity through use of large‒bore capillaries is briefly discussed.
